Name: high-resolution measurement of odor-driven behavior in drosophila larvae
Uploaded: 2008-01-03T21:20:00
Description: Watch this Scientific Journal Video about High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae at JoVE.com

We are grateful to T. Huber and T.P. Sakmar for helpful discussions. This work was supported by grants from the US National Institutes of Health to L.B.V. and the Belgian-American Educational Foundation and the Revson Foundation to M.L.

1. Equipment and Reagents
Odor dilutions:
<ul>
<li>Paraffin oil (Sigma, CAS number: 8012-95-1)</li>
<li>Odors of the highest purity available which elicits attraction in Drosophila larvae1. In this video article, animals were tested with isoamyl acetate(Sigma, CAS number: 123-92-2). </li>
<li>1.5ml glass vials with Teflon cap (Agilent Technologies)</li>
<li>Digital measuring scale for precise dilution by weight</li>
</ul>
Assay arena set-up:
<ul>
<l...

<ol>
	<li>Fishilevich, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemotaxis+behavior+mediated+by+single+larval+olfactory+neurons+in+Drosophila.">Chemotaxis behavior mediated by single larval olfactory neurons in Drosophila.</a> Curr Biol. 15, 2086-2096 (2005).</li><li>Louis, M., Huber, T., Benton, R., Sakmar, T. P., Vosshall, L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bilateral+olfactory+sensory+input+enhances+chemotaxis+behavior.">Bilateral olfactory sensory input enhances chemotaxis behavior.</a> Nature Neuroscience advance online publication. , (2007).</li></ol>

high-resolution measurement of odor-driven behavior in drosophila larvae

Olfactory responses in Drosophila larvae have been traditionally studied in Petri dishes comprising a single peripheral odor source. In this behavioral paradigm, the experimenter usually assumes that the rapid diffusion of odorant molecules from the source leads to the creation of a stable gradient in the dish. To establish a quantitative correlation between sensory inputs and behavioral responses, it is necessary to achieve a more thorough characterization of the odorant stimulus conditions. In this video article, we describe a new method allowing the construction of odorant gradients with stable and controllable geometries. We briefly illustrate how these gradients can be used to screen for olfactory defects (full and partial anosmia) and to study more subtle features of chemotaxis behavior.

Watch this Scientific Journal Video about High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae at JoVE.com

High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

In this video article, we describe a new method allowing the construction of odorant gradients with stable and controllable geometries. We briefly illustrate how these gradients can be used to screen for olfactory defects (full and partial anosmia) and to study more subtle features of chemotaxis behavior.

Olfactory responses in Drosophila larvae have been traditionally studied in Petri dishes comprising a single peripheral odor source. In this behavioral ...

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