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Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

2.6K Views

07:40 min

April 28th, 2022

April 28th, 2022

2,624 Views

0:05

Introduction

1:03

Scar like Tissue in A Dish (SCAD) Preparation

2:50

SCAD: Tissue Culture & Live Imaging of SCADs

4:23

Tissue Harvesting and 2D/3D Immunofluorescence Staining

5:07

Results: An Ex Situ Skin Scarring Assay to Visualize Scar Development

6:43

Conclusion

Transcript

Previously used in vitro or ex vivo models lack dermal cell components and complexity of skin tissue. This ex situ SCAD assay overcomes a set limitation and enables studying the development of scars outside of wounded animal. The SCAD assay allows

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This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar formation.

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