Journal

Medicine

Dissection techniques and histological sampling of the heart in large animal models for cardiovascular diseases

心血管疾病大型动物模型中心脏的解剖技术和组织学取样

5.6K Views

•

10:53 min

•

June 16th, 2022

DOI :

10.3791/63809-v

June 16th, 2022

•

5,575 Views

Irina Constantin¹, Alexandru Flaviu Tăbăran¹

¹Department of Pathology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Chapters

0:04

Introduction

0:59

Necropsy Protocol

4:16

Dissection Protocol

6:21

Sampling Protocol for Histology

7:53

Sampling of Coronary Arteries

8:40

The Four Chambers/Echocardiographic Plane Dissection Technique of the Heart

9:46

Results: Visualization and Histological Examination of the Heart

10:13

Conclusion

Transcript

物种和个体之间心脏的结构差异以及文献的差异需要对心脏解剖学有透彻的了解并遵守严格的方法。该技术可以提高观察者的均匀性，增加实验性心血管疾病研究的可比性和可重复性。不熟悉这种技术的人可能难以识别心脏的所有组成部分。

我的建议是花一些时间熟悉心脏的解剖结构。由于心脏的复杂解剖结构难以理解，因此需要对该方法进行视觉呈现。首先，在将对象动物放在其左侧的尸检台上之前，对对象动物进行称重。

然后通过检查和触诊皮肤和粘膜对受试者进行外部检查，注意颜色、稠度和体积的所有变化。接下来

Summary

由于解剖结构复杂，心脏样本的一致可重复解剖和收集方案的实施可能具有挑战性。本手稿介绍了一些标准心脏夹层方案的关键要素，重点介绍了大体检查方法和通常用于组织病理学检查的采样部位。

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