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2.3K Views
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09:47 min
August 12th, 2022
DOI :
10.3791/63918-v
Chapters
0:05
Introduction
0:56
Myograph Unit Preparation
2:03
Isolation and Mounting of the Aortic Segments onto the Myograph Chamber
3:24
Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings
5:18
Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation
6:25
Contribution of the Endothelial Layer to Aortic Vasorelaxation
8:00
Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model
9:06
Conclusion
Transcript
我们展示了等距或张力肌图作为金标准方案,以测量在良好控制的生理条件下小阻力或大导管血管的反应性。测量结果具有高度可重复性和可靠性。该技术在允许研究人员分别研究不同血管层或同时研究血管壁不同层之间的相互作用方面非常有价值。
演示该程序的将是我实验室的研究实验室专家罗伯特·福克(Robert Folk)。首先打开水浴并将其设置为 37 摄氏度。将两个适当标记的烧杯放入水浴中,一个装有 600 毫升 HEPES-PSS 溶液,另一个装有 150 毫升高钾溶液。
将装有 3
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Summary
本协议描述了使用多腔肌图系统在小鼠主动脉内皮功能的实验 离体 评估中的张力肌图技术的概念和技术应用。
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