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2.4K Views
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05:41 min
December 16th, 2022
DOI :
10.3791/63921-v
Chapters
0:05
Introduction
0:30
Preparation Before Surgery
1:37
Left Anterior Descending Coronary Artery (LAD Ligation)
4:33
Results: Validation of LAD Ligation and Quantification of Infarct Size and Area at Risk
5:18
Conclusion
Transcript
缺血性心脏病是全球死亡的主要原因。该手术可用于诱导心脏缺血再灌注损伤。该协议旨在准确、安全和一致地连接 LAD。
要准备盐水湿棉球,请捏掉一小部分无菌棉,反复滚动以形成球。将棉球浸入无菌的0.9%盐水中,挤出多余的盐水。将棉球储存在用75%乙醇灭菌的干净盒子中。
要准备固定钩,请用75%乙醇对夹子和橡皮筋进行消毒。要准备结扎环,请将 7-0 丝线的中间放在锥形非锻造手术针的半圆尺寸三弹簧眼中。要准备圈套控制器,请使用剪刀切割 5 毫米聚乙烯 10 管。
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Summary
在本文中,我们提出了一个专注于左前降冠状动脉结扎术的协议,通过技术上修改大鼠的传统程序进行急性心肌缺血再灌注研究。
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