June 23rd, 2022
•This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent.
Tags
Related Videos
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved