Subcutaneous Injection of Human Hepatocellular Carcinoma Cell Lines
1:45
Orthotopic Liver Implantation and Hepatic Artery Ligation
4:00
[18F] FMISO and [18F] FDG Injection
4:42
PET/MR Acquisition
5:57
Results: Effect of Hepatic Artery Ligation on Orthotopic Hepatocellular Carcinoma in Mice
7:02
Conclusion
Transcript
Our methods creates a reliable hypoxic hepatocellular carcinoma model for noninvasive imaging of tumor hypoxia to study HCC biology in vivo, which can better recapitulate cancer development in humans. Our reliable HCC model together with a highly-
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Here, we present a protocol to create orthotopic hepatocellular carcinoma xenografts with and without hepatic artery ligation and perform non-invasive positron emission tomography (PET) imaging of tumor hypoxia using [18F]Fluoromisonidazole ([18F]FMISO) and [18F]Fluorodeoxyglucose ([18F]FDG).