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DOI :
10.3791/63998-v
May 20th, 2022
Chapters
0:04
Introduction
1:19
Standard Immunostaining Procedure of Thinly Sliced Brain Sections on Slides
3:36
Alternate Processing of Lineage-Traced Brains by iDISCO Brain Clearing and Whole Mount Imaging
6:53
Imaging of Stained Slides or Cleared Brains
7:27
Results: Lineage Tracing of Adult mTert-rtTA::oTet-Cre::Rosa-mTmG Mouse Brains
9:04
Conclusion
使用诱导的转基因谱系追踪小鼠系,用荧光团永久标记干细胞及其后代的能力允许对 体内的活化,增殖,迁移和/或分化进行空间和时间分析。谱系追踪可以揭示有关谱系承诺、对干预的反应和多能性的新信息。
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