Isolation of Mediastinal Lymph Nodes, Lungs, Mesenteric Lymph Node Chains, and Small Intestine
2:37
Preparation of Single-Cell Suspensions from the Small Intestine, Lung, and Lymph Nodes
6:20
Results: Flow Cytometry Analysis and Gating Strategy for the Identification of Th9 Cells and IL-9+ ILC2 Cells
7:48
Conclusion
Transcript
This protocol allows for the isolation and characterization of lymphoid cells expressing IL-9 in N.brasiliensis non-lymphoid infected tissues including the infected cut which recently was technically invisible.This protocol also provides an altern
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IL-9-ekspressive T- og ILC2-celler induceres under N. brasiliensis-infektion , men deres karakterisering er stort set blevet overset i den inficerede tarm på grund af deres lavfrekvente og differentielle kinetik. Denne protokol beskriver isoleringen af disse celler fra forskellige målorganer og bekræftelse af deres identitet via flowcytometri på forskellige infektionsstadier.