Preparation of Cultured Tumor Cells for Implantation
2:33
Tumor Implantation
3:19
Vaccine Preparation and Injection of Flt3L-Expressing B16 F10 (B16-Flt3) Cells
4:20
Intratumoral Immune Cell Isolation
6:59
Results: Using a Cell-Based Tumor Vaccine Expressing Flt3 Model to Study Intratumoral Immune Cell Infiltrates
8:45
Conclusion
Transcript
This protocol provides with clinical solid tumor immunotherapy model and a research platform to study the relationship between tumor cells and infiltrating immune cells. This cell-based tumor vaccine is convenient, straightforward to use, and can
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The protocol presents a cancer immunotherapy model using cell-based tumor vaccination with Flt3L-expressing B16-F10 melanoma. This protocol demonstrates the procedures, including preparation of cultured tumor cells, tumor implantation, cell irradiation, measurement of tumor growth, isolation of intratumoral immune cells, and flow cytometry analysis.