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DOI :
10.3791/64092-v
December 9th, 2022
Chapters
0:04
Introduction
0:45
Preparation of Lipid‐Conditioned Bacteria
2:00
Lipid Supplementation for Caenorhabditis elegans
3:37
RNA Extraction from a Small Number of Whole Worms
5:53
Results: Validation of Transcriptional Changes, Transcriptional Evaluations, and Lifespan Assay Upon Lipid Supplementation
6:41
Conclusion
本プロトコルは、 カエノラブディティスエレガンスの液体およびオンプレート培養における脂質補給方法を、バルクまたは少数のワームおよびワーム組織からの縦断的研究および遺伝子転写分析と組み合わせて説明しています。
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