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DOI :
10.3791/64092-v
December 9th, 2022
Chapters
0:04
Introduction
0:45
Preparation of Lipid‐Conditioned Bacteria
2:00
Lipid Supplementation for Caenorhabditis elegans
3:37
RNA Extraction from a Small Number of Whole Worms
5:53
Results: Validation of Transcriptional Changes, Transcriptional Evaluations, and Lifespan Assay Upon Lipid Supplementation
6:41
Conclusion
본 프로토콜은 Caenorhabditis elegans에 대한 액체 및 온플레이트 배양에서 지질 보충 방법을 설명하고 벌크 또는 소수의 웜 및 웜 조직으로부터의 종단 연구 및 유전자 전사 분석과 결합합니다.
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