JoVE Logo
Faculty Resource Center

Sign In

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing (Video) | JoVE

DOI :

10.3791/64114-v

June 16th, 2022

June 16th, 2022

3,454 Views

1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.

-- Views

Related Videos

article

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model (Video) | JoVE

article

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae (Video) | JoVE

article

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation (Video) | JoVE

article

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration (Video) | JoVE

article

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli (Video) | JoVE

article

Development of Leishmania Species Strains with Constitutive Expression of eGFP (Video) | JoVE

article

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations (Video) | JoVE

article

Author Spotlight: RNAi Inheritance and ChIP in C. elegans (Video) | JoVE

article

Exercise Test for Evaluation of the Functional Efficacy of the Pig Cardiovascular System (Video) | JoVE

article

Ligation and Decrosslinking of Scarce Cell DNA (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved