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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

2.1K Views

08:19 min

September 28th, 2022

September 28th, 2022

2,121 Views

0:05

Introduction

0:33

Target Design and In Vitro Synthesis of sgRNA

1:18

Embryo Collection and Preparation

2:39

Microinjection of Embryos

4:24

Post-Injection Insect Rearing

5:07

Mutant Screening

6:41

Results: Mutant Screening in B. dorsalis

7:39

Conclusion

Transcript

The oriental fruit fly is a highly invasive and adaptive pest species. Effective methods for functional gene research could help us to develop environmental-friendly pest management strategies. This method is high efficiency and low cost.

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This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

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