JoVE Logo
Faculty Resource Center

Sign In

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

DOI :

10.3791/64195-v

September 28th, 2022

September 28th, 2022

1,889 Views

1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

Tags

CRISPR Cas9

-- Views

Related Videos

article

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

article

Isolation and Biophysical Study of Fruit Cuticles

article

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

article

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

article

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

article

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

article

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

article

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

article

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

article

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved