Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
2:33
MALDI Matrix Application via Robotic Spraying
3:12
Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
4:37
Imaging Mass Spectrometry Data Analysis and Compound Identification
5:57
Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
7:45
Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
8:08
Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
9:09
Conclusion
Transcript
Our protocol enables the visualization of spatial metabolism of microbial interactions that occur during infection. We have developed this imaging mass spectrometry workflow for bacterial co-cultures grown on agar and tissue models. Analyzing non-
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A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.