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1.1K Views
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07:02 min
July 12th, 2022
DOI :
10.3791/64273-v
Chapters
0:04
Introduction
0:41
Tools and Holder Customization
2:29
Embryo Dissection
3:50
Microscope Preparation, Embryo Mounting, and Image Acquisition
5:56
Results: 3D Live Imaging of Cardiac Progenitor Cells in the Mouse Embryo
6:35
Conclusion
Transcript
该协议允许在小鼠胚胎发育时对其进行成像。这样可以详细研究早期心脏发生。与拍摄假胚胎的静态图像一样,实时成像可以完全访问研究胚胎发育中的动态过程。
生命成像所需的技能很难从仅文本出版中掌握。看着另一个人这样做对于学习至关重要。首先,切割一厘米长的钨丝片,并通过将钨丝尖端浸入饱和硝酸钠溶液中,将它们磨尖至直径0.02至0.05毫米。
要准备弯头玻璃毛细管,请将标准一毫米玻璃毛细管的中点放在本生打火机上三到六秒钟,从两端缓慢拉动,直到毛细管变薄且有弹性。然后,将毛细管分成
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Summary
我们提出了小鼠胚胎培养和成像的详细方案,可实现心脏祖细胞的3D +时间成像。该视频工具包解决了成功进行实时成像所需的关键技能,否则很难从纯文本出版物中获得。
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