Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
1.9K Views
•
06:02 min
August 4th, 2022
DOI :
10.3791/64282-v
Chapters
0:04
Introduction
0:40
Drosophila Crossing and Egg Laying
1:54
Amino Acid Starvation Induces Autophagy
2:51
Third Instar Larvae Dissection and Sample Tissue Processing
4:29
Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
5:22
Conclusion
Transcript
该协议描述了如何通过氨基酸消耗在黑腹果蝇幼虫脂肪体中诱导自噬,以及如何分析突变克隆之间自噬的差异。由于自噬对培养条件下的营养可用性高度敏感,克隆分析是一种分析同一组织中突变细胞与野生型细胞的方法,在解剖自噬缺陷方面具有优势。首先将3只雄性和15只雌性成年果蝇引入带有标准玉米面或糖蜜或琼脂果蝇培养基的培养瓶中,在25摄氏度下进行交配。
在诱导后 48 小时,轻拍交配小瓶,直到苍蝇被击晕并落到小瓶底部的培养基上。拔下交配小瓶的插头,用未插拔的倒置培养瓶用新鲜培养基盖住其嘴。然后翻转
Sign in or start your free trial to access this content
Summary
本协议描述了通过营养消耗在黑腹果蝇幼虫脂肪体中诱导自噬,并使用转基因苍蝇菌株分析自噬的变化。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved