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1.4K Views
•
07:13 min
February 10th, 2023
DOI :
10.3791/64286-v
Chapters
0:04
Introduction
1:04
Induction of Noise‐Induced Hearing Loss (NIHL) in Mice
3:07
Auditory Brainstem Response (ABR)‐Based Assessment of Hearing Threshold
4:19
Microscope Examination
5:18
Results: Assessing Hearing Function and Outer Hair Cell Damage in NIHL Mice
6:32
Conclusion
Transcript
尽管已经有关于噪音引起的听力损失的研究,但没有一个如此详细地记录该方法,使其成为一个重要的程序。该方法有效地引起小鼠噪声引起的听力损失,并通过ABR观察听力阈值的实际变化。噪声性听力损失的动物模型有助于科学家彻底了解噪声性听力损失的机制,进而优化相应的治疗策略。
演示该程序的将是Dian-Shiue Lee,他是我实验室的一位非常有才华的学生。为了准备实验小鼠的笼子,使用捕鼠笼并将四块瓦楞塑料板切成适当的尺寸,使它们适合笼子。为了防止小鼠的脚被网状网格割伤,请分别将其中两块放在
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Summary
在这里,我们提出了噪声诱导听力损失(NIHL)小鼠模型的方案。为了诱导NIHL,我们开发了一种使用波纹塑料,捕鼠笼和扬声器的新型简单设备。采用听觉脑干反应和免疫荧光成像分别评估听力功能和外毛细胞损伤。
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