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DOI :
10.3791/64290-v
February 3rd, 2023
Chapters
0:04
Introduction
1:02
Heart Dissection
2:07
Preparation of Cardiac Cell Suspension
4:24
Results: Physical and Molecular Attributes of Cardiac Cells
5:07
Conclusion
ここでは、心筋梗塞後(MI)マウスの心臓から十分な量の生存率の高い単一の非心筋細胞を単離するためのプロトコルについて説明します。これは、その後のシングルセルシーケンシング、フローサイトメトリー解析、および初代細胞培養に使用できます。
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