Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
2.6K Views
•
10:23 min
January 20th, 2023
DOI :
10.3791/64308-v
Chapters
0:05
Introduction
1:04
Stimulating the Cells
2:05
Collecting the Combined Supernatant and Protein Lysate to Be Used for Caspase Western Blots
3:20
Performing Western Blotting Using the Lysates Collected from the BMDMs or Tissue Homogenates
7:55
Results: Monitoring Caspase Cleavage and Activation
9:31
Conclusion
Transcript
该协议提供了对细胞死亡过程激活的多方面理解,这可以提供对疾病机制的关键见解并为治疗策略提供信息。该协议依赖于使用更简单的技术,并从单个内源性细胞群中获取多个半胱天冬酶的激活,以极大地确定激活。先天免疫细胞死亡与整个疾病谱有关,从感染到炎症性疾病再到癌症。
通过半胱天冬酶活化了解细胞死亡的分子机制可以为疾病过程提供重要的见解。来自实验室的博士后研究助理Julieann博士将演示该程序。分离骨髓并分化BMDM后,用甲型流感病毒感染细胞。
计算 20 个斑块形成单位的多重
Sign in or start your free trial to access this content
Summary
该协议描述了一种评估半胱天冬酶活化(半胱天冬酶-1,半胱天冬酶-3,半胱天冬酶-7,半胱天冬酶-8,半胱天冬酶-9和半胱天冬酶-11)的综合方法,以响应 体外 和 体内 (小鼠)感染,无菌侮辱和癌症模型,以确定细胞死亡途径的启动,例如焦亡,细胞凋亡,坏死性凋亡和全细胞凋亡。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved