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3.4K Views
•
06:49 min
October 6th, 2022
DOI :
10.3791/64326-v
Chapters
0:04
Introduction
0:50
Sperm Collection by Abdominal Massage
2:14
Sperm Collection by Testes Dissection
3:02
Computer-Assisted Sperm Analysis (CASA)
4:33
Results: Determination of Sperm Motility Under Various Factors
6:11
Conclusion
Transcript
这种技术能够对小型模式鱼日本马达卡的小米进行取样,并以一致的方式调查其质量。特别是通过腹部按摩收集精子允许对同一条鱼重复采样,因为这种方法不是侵入性的。此外,该技术可应用于马达卡精子冷冻保存。
精子质量是受精成功的重要指标,因此是生态和整体生产生理学研究中分析的重要参数。首先,准备一块柔软的保持海绵,将其切成适合培养皿。在海绵的中间切一条直线,这条直线足够长,可以将鱼的腹侧向上。
麻醉鱼后,用纸巾轻轻擦拭鱼的腹部。将鱼放在潮湿的保持海绵的槽中,腹侧朝上,使其鳃暴露于
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Summary
本文介绍了两种从小型模式鱼青鳉鱼(Oryzias latipes)收集精子的快速有效方法,以及使用计算机辅助精子分析(CASA)可靠地评估精子质量的方案。
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