Name: evaluation of fluid overload by bioelectrical impedance vectorial analysis
Uploaded: 2022-08-17T14:40:00
Description: Watch this Scientific Journal Video about Evaluation of Fluid Overload by Bioelectrical Impedance Vectorial Analysis at JoVE.com

作者要感谢教授。意大利帕多瓦大学医学和外科科学系的Piccoli和Pastori提供BIVA软件。这项研究没有从公共、商业或非营利部门的资助机构获得任何具体资助。

以下协议获得批准（REF. 3057），并遵循SZ国家医学与营养研究所人类研究伦理委员会的指导方针。此外，本研究事先获得了患者的同意。
注意：该程序将用于使用四极多频设备测量生物电阻抗分析（见 材料表），并将在50 kHz的单个频率下提供精确的电阻和电抗值，以及200 kHz和5 kHz阻抗值（200/5 kHz）之间的比率。
1. 测试前
<ol><li>...

<ol>
	<li>da Silva, A. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Association+of+hyperhydration+evaluated+by+bioelectrical+impedance+analysis+and+mortality+in+patients+with+different+medical+conditions:+Systematic+review+and+meta-analyses.+Clinical+Nutrition+ASPEN+Association+of+hyperhydration+evaluated+by+bioelectrical.">Association of hyperhydration evaluated by bioelectrical impedance analysis and mortality in patients with different medical conditions: Systematic review and meta-analyses. Clinical Nutrition ASPEN Association of hyperhydration evaluated by bioelectrical.</a> Clinical Nutrition ESPEN. 28, 12-20 (2018).</li><li>Kammar-Garc&#237;a, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+Bioelectrical+Impedance+Analysis+parameters+for+the+detection+of+fluid+overload+in+the+prediction+of+mortality+in+patients+admitted+at+the+emergency+department.">Comparison of Bioelectrical Impedance Analysis parameters for the detection of fluid overload in the prediction of mortality in patients admitted at the emergency department.</a> Journal of Parenteral and Enteral Nutrition. 45 (2), 414-422 (2021).</li><li>Kammar-Garc&#237;a, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SOFA+score+plus+impedance+ratio+predicts+mortality+in+critically+ill+patients+admitted+to+the+emergency+department:+Retrospective+observational+study.">SOFA score plus impedance ratio predicts mortality in critically ill patients admitted to the emergency department: Retrospective observational study.</a> Healthcare (Basel). 10 (5), 810 (2022).</li><li>Frank Peacock, W., Soto, K. 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C., Piccoli, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioelectrical+Impedance+Vector+Analysis+for+Assessment+of+Hydration+in+Physiological+States+and+Clinical+Conditions.">Bioelectrical Impedance Vector Analysis for Assessment of Hydration in Physiological States and Clinical Conditions.</a> Handbook of Anthropometry. , 287-305 (2012).</li><li>Piccoli, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bivariate+normal+values+of+the+bioelectrical+impedance+vector+in+adult+and+elderly+populations.">Bivariate normal values of the bioelectrical impedance vector in adult and elderly populations.</a> The American Journal of Clinical Nutrition. 61 (2), 269-270 (1995).</li><li>Roubenoff, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+bioelectrical+impedance+analysis+to+elderly+populations.">Application of bioelectrical impedance analysis to elderly populations.</a> The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. 52 (3), 129-136 (1997).</li><li>Espinosa-Cuevas, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bio+impedance+vector+an&#225;lisis+for+body+composition+in+Mexican+population.">Bio impedance vector an&#225;lisis for body composition in Mexican population.</a> Revista de Investigaci&#243;n Cl&#237;nica. 59 (1), 15-24 (2007).</li><li>Demirci, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impedance+ratio:+a+novel+marker+and+a+power+predictor+of+mortality+in+hemodialysis+patients.">Impedance ratio: a novel marker and a power predictor of mortality in hemodialysis patients.</a> International Urology and Nephrology. 48 (7), 1155-1162 (2016).</li><li>Plank, L. D., Li, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioimpedance+illness+marker+compared+to+phase+angle+as+a+predictor+of+malnutrition+in+hospitalized+patients.">Bioimpedance illness marker compared to phase angle as a predictor of malnutrition in hospitalized patients.</a> Clinical Nutrition. 32, 85 (2013).</li><li>Castillo-Martinez, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioelectrical+impedance+and+strength+measurements+in+patients+with+heart+failure:+comparison+with+functional+class.">Bioelectrical impedance and strength measurements in patients with heart failure: comparison with functional class.</a> Nutrition. 23 (5), 412-418 (2007).</li><li>Earthman, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Body+composition+tools+for+assessment+of+adult+malnutrition+at+the+bedside:+A+tutorial+on+research+considerations+and+clinical+applications.">Body composition tools for assessment of adult malnutrition at the bedside: A tutorial on research considerations and clinical applications.</a> Journal of Parenteral and Enteral Nutrition. 39 (7), 787-822 (2015).</li><li>Piccoli, A., Pastori, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=BIVA+software.">BIVA software.</a> Department of Medical and Surgical Sciences. , (2002).</li><li>Basso, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluid+management+in+the+intensive+care+unit:+bioelectrical+impedance+vector+analysis+as+a+tool+to+assess+hydration+status+and+optimal+fluid.">Fluid management in the intensive care unit: bioelectrical impedance vector analysis as a tool to assess hydration status and optimal fluid.</a> Blood Purification. 36 (3-4), 192-199 (2013).</li><li>Piccoli, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioelectrical+impedance+measurement+for+fluid+status+assessment.">Bioelectrical impedance measurement for fluid status assessment.</a> Contributions to Nephrology. 164, 143-152 (2010).</li><li>National Institutes of Health Technology. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioelectrical+impedance+analysis+in+body+composition+measurement:+National+Institutes+of+Health+Technology+Assessment+Conference+Statement.">Bioelectrical impedance analysis in body composition measurement: National Institutes of Health Technology Assessment Conference Statement.</a> The American Journal of Clinical Nutrition. 64, 524-532 (1996).</li><li>Silva, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lack+of+agreement+of+in+vivo+raw+bioimpedance+measurements+obtained+from+two+single+and+multifrequency+bioelectrical+impedance+devices.">Lack of agreement of in vivo raw bioimpedance measurements obtained from two single and multifrequency bioelectrical impedance devices.</a> European Journal of Clinical Nutrition. 73 (7), 1077-1083 (2019).</li><li>Mulasi, U., Kuchnia, A. J., Cole, A. J., Earthman, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioimpedance+at+the+bedside:+current+applications,+limitations,+and+opportunities.">Bioimpedance at the bedside: current applications, limitations, and opportunities.</a> Nutrition in Clinical Practice. 30 (2), 180-193 (2015).</li><li>Chabin, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioimpedance+analysis+is+safe+in+patients+with+implanted+cardiac+electronic+devices.">Bioimpedance analysis is safe in patients with implanted cardiac electronic devices.</a> Clinical Nutrition. 38 (2), 806-811 (2019).</li><li>Gonz&#225;lez-Correa, C. H., Caicedo-Eraso, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioelectrical+impedance+analysis+(BIA):+a+proposal+for+standardization+of+the+classical+method+in+adults.">Bioelectrical impedance analysis (BIA): a proposal for standardization of the classical method in adults.</a> Journal of Physics: Conference Series. 47, 407 (2012).</li><li>Di Somma, S., Gori, C. S., Grandi, T., Risicato, M. G., Salvatori, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluid+assessment+and+management+in+the+emergency+department.">Fluid assessment and management in the emergency department.</a> Contributions to Nephrology. 164, 227-236 (2010).</li><li>Kammar-Garc&#237;a, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mortality+in+adult+patients+with+fluid+overload+evaluated+by+BIVA+upon+admission+to+the+emergency+department.">Mortality in adult patients with fluid overload evaluated by BIVA upon admission to the emergency department.</a> Postgraduate Medical Journal. 94 (1113), 386-391 (2018).</li></ol>

值得一提的是，已发表的文献中提出了不同的生物电阻抗分析（BIA）方法，包括使用1-500 kHz的多频率（MF-BIA），50 kHz的相位敏感单频（SF-BIA）和5 kHz至2 MHz的光谱BIA。 关于单频和多频BIA设备的协议，研究提供了不一致的结果6，包括源电流、频率、电流在指定容差范围内的总阻抗范围、分辨率和显示阻抗的精度，如美国国立卫生研究院 （NIH） 技术评估会议声明21...

液体超负荷 （FO） 定义为体液过量或一个或多个液体隔室相对过量 1，常见于危重患者，与较高的发病率和死亡率相关 1，2，3。水合状态的改变范围很广;可提示肾、心脏或肝衰竭;和/或可能是过量口服摄入或医源性错误的结果4.在急诊科，常规评估水合状态具有挑战性，因为放射性同位素体积测量的金标准需要专门的技术，既昂贵又耗时，并且可能无法识别水合状态的早期干扰。因此，通常使用其他不太准确的方法，包括临床检查和累积体液平衡（24小时内以mL为单位的体积）5。准确灵敏地测定液体容量状态对于帮助临床医生控制体液、管理静脉输液和维持血流动力学稳定是必要的，从而使患者能够接受早期治疗3，5，6。容量评估中的错误可导致缺乏必要的治疗或实施不必要的治疗，例如过量补液，这两者都与住院费用增加、并发症和死亡率增加有关4。
最近人们对生物电阻抗分析（BIA）的兴趣日益浓厚，它被认为是对个体水合状态进行分类的替代方法。BIA是一种安全，无创，便携，快速，床边且易于使用的方法，旨在估计身体隔间成分。该分析测量软组织通过放置在手和脚上的四个表面电极对注入体内的交流电（800μA）流动产生的反对。BIA估计的体内总水分已被证明与通过氘稀释获得的水分具有高度相关性（r = 0.93，p = 0.01）7。
相敏 BIA 器件评估相位角和阻抗 （Z 50） 的直接测量，在单频模式 （50 kHz） 或多频模式（5 kHz 至 200 kHz）下获得电阻 （R） 和电抗 （Xc）8。将R和Xc值除以受试者的高度（以m为单位）的平方，以控制导体长度的个体间差异，并将它们绘制在R-Xc图中是生物电阻抗矢量分析（BIVA）中用于估计流体状态的方法。BIVA是一种替代阻抗方法，由Piccoli等人开发9，它使用R（即，反对通过细胞内和细胞外离子溶液的交流电流动）与Xc之间的空间关系来评估软组织水合作用，独立于在有限和特定样品中生成的多元回归预测方程10.因此，液体状态的分类比全身水分的量化更精确和准确。受试者的R和Xc值产生一个向量，其位置可以相对于R-Xc图中的公差区间进行评估，这可以解释为指示正常或异常的水合作用，基于从健康参考人群11，12，13得出的平均向量的距离。
在之前的一项研究中，我们比较了用于检测急诊科 （ED） 患者液体超负荷和死亡率预测的不同生物电阻抗分析参数，并证明 BIVA（相对风险 = 6.4;95% 置信区间从 1.5 到 27.9;p = 0.01）和阻抗比（相对风险 = 2.7;95% 置信区间从 1.1 到 7.1;p = 0.04）改善了对 30 天死亡率概率的估计3。
流体过载也可以使用阻抗比（imp-R）来估计，阻抗比是多频生物电阻抗设备在200 kHz处测量的阻抗与在5 kHz处测量的阻抗之间的比率。Imp-R考虑了全身水（Z200）和细胞外水液空间（Z5）中的传导。电流渗透到电池中是与频率相关的，200/5 kHz 比率描述了较大与较小的电流进入电池3，8 的比率。如果这两个值之间的差异随着时间的推移而减小，则可能表明细胞变得不那么健康14。
在健康个体中观察到男性的 Imp-R 值为 ≤0.78，女性为 ≤0.8215。接近 1.0 的值表示两种阻抗彼此更接近，并且身体细胞不太健康。在危重疾病的情况下，细胞膜在5kHz时的电阻降低，5和200kHz的阻抗值之间的差异明显降低，表明细胞恶化3。值> 1.0 表示设备错误16，17。因此，本研究的目的是展示如何通过生物电阻抗矢量分析以及使用阻抗比来评估流体过载的存在，在急诊科入院的患者中使用四极多频设备测量。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Alcohol 70% swabs</td>
			<td>NA</td>
			<td>NA</td>
			<td>Any brand can be used</td>
		</tr>
		<tr>
			<td>BIVA software 2002</td>
			<td>NA</td>
			<td>NA</td>
			<td>Is a sofware created for academic use, can be download in http://www.renalgate.it/formule_calcolatori/bioimpedenza.htm in &#34;LE FORMULE DEL Prof. Piccoli&#34; section</td>
		</tr>
		<tr>
			<td>Chlorhexidine Wipes</td>
			<td>NA</td>
			<td>NA</td>
			<td>Any brand can be used</td>
		</tr>
		<tr>
			<td>Examination table</td>
			<td>NA</td>
			<td>NA</td>
			<td>Any brand can be used</td>
		</tr>
		<tr>
			<td>Leadwires square socket</td>
			<td>BodyStat</td>
			<td>SQ-WIRES</td>
			<td></td>
		</tr>
		<tr>
			<td>Long Bodystat 0525 electrodes</td>
			<td>BodyStat</td>
			<td>BS-EL4000</td>
			<td></td>
		</tr>
		<tr>
			<td>Quadscan 4000 equipment</td>
			<td>BodyStat</td>
			<td>BS-4000</td>
			<td>Impedance measuring range: 20 - 1300 &#937; ohms 
			Test Current: 620 &#956;A 
			Frequency: 5, 50, 100, 200 kHz 
			Accuracy: Impedance 5 kHz: +/- 2 &#937; 
			Impedance 50 kHz: +/- 2 &#937; 
			Impedance 100 kHz: +/- 3 &#937; 
			Impedance 200 kHz: +/- 3 &#937; 
			Resistance 50 kHz: +/- 2 &#937; 
			Reactance 50 kHz: +/- 1 &#937; 
			Phase Angle 50 kHz: +/- 0.2&#176; 
			Calibration: A resistor is supplied for independent verification from time to time. The impedance value should read between 496 and 503 &#937;.</td>
		</tr>
	</tbody>
</table>

evaluation of fluid overload by bioelectrical impedance vectorial analysis

早期发现和管理液体超负荷在急性疾病中至关重要，因为治疗干预的影响可导致死亡率降低或增加。准确的液体状态评估需要适当的治疗。不幸的是，由于放射性同位素液测量的金标准方法昂贵、耗时且在急性护理临床环境中缺乏敏感性，因此通常使用其他不太准确的方法，例如临床检查或 24 小时输出。生物电阻抗矢量分析（BIVA）是一种基于阻抗的替代方法，其中绘制受试者的原始参数电阻和电抗以产生矢量，其位置可以相对于R-Xc图中的公差区间进行评估。然后，根据与健康参考人群得出的平均向量的距离，将液体状态解释为正常或异常。本研究的目的是演示如何通过生物电阻抗矢量分析和用四极多频设备测量的急诊科患者是否存在液体过载。

作为上述方法的一个例子，我们展示了两名急诊科收治的女性的结果。入院时使用相敏多频器件评估生物电阻抗分析（见 材料表），并使用获得的电阻（R）和电抗（Xc）值计算BIVA图。结果显示，水合过度患者的预后较差，SOFA、Charlson指数评分等临床特征与体液超负荷有关。
在图 2 中，用 Δ 绘制的结果表示液体状态正常的 77 岁女性（身高 =...

Watch this Scientific Journal Video about Evaluation of Fluid Overload by Bioelectrical Impedance Vectorial Analysis at JoVE.com

Evaluation of Fluid Overload by Bioelectrical Impedance Vectorial Analysis

在这项研究中，我们演示了如何通过生物电阻抗矢量分析（BIVA）和使用四极多频设备测量的阻抗比来评估急诊科患者是否存在液体过载。BIVA和阻抗比是预测不良结果的可靠且有用的工具。

通过生物电阻抗矢量分析评估流体过载

Early detection and management of fluid overload are critically important in acute illness, as the impact of therapeutic intervention can result in ...

早期准确地发现液体超负荷在急性疾病中至关重要，因为治疗干预的影响可降低或增加死亡率。生物电阻抗矢量分析是一种替代阻抗方法，也是一种用于估计流体状态的方法，独立于在有限和特定样品中生成的多元回归预测方程。液体容量状态的测定对于帮助临床医生控制体液、管理静脉输液和维持血流动力学稳定是必要的，从而使患者能够早期接受治疗。该方法适用于所有液体超负荷的慢性患者，例如心脏、肾脏或肝衰竭。演示该程序的将是我实验室的研究生Yunuen Reyes Paz。首先，使用洗必泰湿巾清洁设备，然后洗手。如果设备的屏幕显示图例更换电池，则更换电池。如果患者有意识，请向他们解释程序。获取患者的年龄和精确身高测量值（以厘米为单位），并将这些数据引入设备。从右脚上取下鞋子和袜子，以及患者佩戴的任何金属物品，如手表或手镯。在进行测量之前，将患者置于仰卧位五分钟，双腿和手臂以 45 度角展开，验证他们没有与身体的任何其他部位接触。用 70% 酒精垫清洁电极放置的表面两次。在手腕上突出的骨头之间画一条假想线，并将两个电极放在右手背侧，每个电极位于该线的中心。将两个电极放在右脚上，一个在第三跖骨指骨关节后面，一个在踝关节的跗骨关节后面，在内踝和外踝之间。要放置电极，请沿着下面的骨骼。使用最靠近指甲的红色鳄鱼夹和最靠近脚踝或手腕的黑色夹将引线连接到设备。确保电线之间没有交叉。确保患者在测量过程中没有说话或移动，因为这会影响结果。患者的ID将出现在屏幕上。按照文本手稿中的说明滚动并更改患者的参数。确保电极正确卡住，然后按回车键。该窗口将在屏幕上指示测量。测量大约需要 6 到 10 秒，测量完成后会发出哔哔声。设备将在四个不同的频率下显示原始阻抗值。5、50、100 和 200 千赫兹，以及 50 千赫兹时的电阻和电抗，这是对液体超负荷患者进行分类所需的值。下载名为BIVA容差RXc图的软件并打开它。观察该软件位于具有七个工作表的电子表格程序的工作簿中。指南、参考总体、点图、路径、主题、Z 分数和 Z 图。右键单击参考总体表。选择所选参考总体的行并将其复制粘贴到第二行中。右键单击“受试者”表，然后在第二行中插入分配给患者的受试者 ID。在名为 Seq 的第二列中，始终输入数字 1，并选择性地填写姓氏和姓名列。在性别列中，输入 F 表示女性，输入 M 表示男性患者。在接下来的两列中，输入电阻和电抗分别为 50 kHz。在接下来的两列中，插入高度（以厘米为单位）和重量（以千克为单位）。在人口代码列中，插入“参考人口”表第一列中显示的数字。在组代码中，随机选择一个介于 1 和 10 之间的数字，并在下一列中插入患者的年龄。在电子表格程序菜单中，转到补码选项卡，然后右键单击计算选项以获取按高度和相位角调整的电阻和电抗值。右键单击点图工作表，观察是否为所选参考总体绘制了 50%、75% 和 95% 容差椭圆。在对话框中，选择组。右键单击位于主题工作表上的组代码中的数字，然后右键单击“确定”。接下来，将显示BIVA图，并将主题矢量显示为几何图形。矢量落在 75% 耐受椭圆下极之外的患者将被归类为液体超负荷。将 200 千赫兹的 Z 除以 5 千赫兹的 Z，分别反映体内总水分和细胞外水室。要获得阻抗比，等于或大于 0.85 的值表示流体过载。用三角形符号表示的图表示体液状态正常的 77 岁女性。生物阻抗结果显示电阻为586.7，电抗为62.1。同时，方形符号表示一名62岁的女性，液体超负荷。生物阻抗结果显示电阻为332.6，电抗为33.6。患者必须处于仰卧位，没有任何金属接触。应将肢体外展到身体皮肤接触处，并放置电极的表面，并应用酒精垫彻底清洁。生物电矢量分析和阻抗比已被证明是检测流体过载存在的有用工具，并且还显示可以预测死亡风险。

Research

JoVE Journal

Medicine

A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells

一个实时的基于电阻抗技术测量癌细胞入侵内皮细胞单层

Metastatic dissemination of malignant cells requires degradation of basement membrane, attachment of tumor cells to vascular endothelium, retraction of ...

科研

医学

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

小鼠模型的腔内MCAO脑梗塞评价甲酚紫染色

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis

视频栅格立体成像和跑步机步态分析对腰椎融合手术后患者体型和步态剖面的评价

This protocol provides guidance on how to perform high resolution video rasterstereography and treadmill gait analysis on patients after lumbar fusion ...

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Rats are widely used in pharmacokinetics (PK) and toxicokinetic (TK) studies that need to collect a certain amount of blood at specific time points to ...

Synovial Fluid Analysis to Identify Osteoarthritis

滑液分析以识别骨关节炎

Synovial fluid (SF) analysis is important in diagnosing osteoarthritis (OA). Macroscopic and microscopic features, including total and differential white ...

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

使用液相色谱-串联质谱分析原发性痛经大鼠的原始和加工的Cyperi Rhizoma样品

Cyperi rhizoma (CR) is widely used in gynecology and is a general medicine for treating women's diseases in China. Since the analgesic effect of CR is ...

Modified Arteriovenous Fistula Surgery to Establish the RV Volume Overload Mouse Model

Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model

Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model (Video) | JoVE 

出生后右心室容积超负荷小鼠模型的建立与确认

Right ventricular (RV) volume overload (VO) is common in children with congenital heart disease. In view of distinct developmental stages,the RV ...

Predicting Prognosis in Acute Heart Failure Using Phase Angle & BIVA Z-Scores

Clinical Application of Phase Angle and BIVA Z-Score Analyses in Patients Admitted to an Emergency Department with Acute Heart Failure

Unveiling Prognostic Indicators in Heart Failure - The Role of Phase Angle and Bioelectrical Impedance Analysis (Video) | JoVE

相位角和BIVA Z评分分析在急诊科急性心力衰竭患者中的临床应用

Acute heart failure is characterized by neurohormonal activation, which leads to sodium and water retention and causes alterations in body composition, ...

Developing an Exercise Program for Rheumatoid Arthritis Patients

Evaluation of Changes in Hydration and Body Cell Mass with Bioelectrical Impedance Analysis after Exercise Program for Rheumatoid Arthritis Patients

Implementation of BIVA for Analyzing Disease Risk Factors in Patients with Low Body Cell Mass (Video) | JoVE

类风湿性关节炎患者运动计划后通过生物电阻抗分析评估水合作用和体细胞质量的变化

Rheumatoid arthritis (RA) is a debilitating disease that can result in complications such as rheumatoid cachexia. While physical exercise has shown ...