PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer
2:19
Coverslip Substrate Preparation
3:34
Transferring the Textured Film to the Final Substrate
4:33
Cell Seeding, Release, and Collection of the Organoids
6:29
Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation
7:00
Conclusion
Transcript
This protocol demonstrate a microtexture cell culture dish engineered for the growth of hundreds of organoids with knowledge of material and fully compatible with microscopy. Our technique efficiently allow to generate thousands of organoids, full
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This paper presents a fabrication protocol for a new type of culture substrate with hundreds of microcontainers per mm2, in which organoids can be cultured and observed using high-resolution microscopy. The cell seeding and immunostaining protocols are also detailed.