January 13th, 2023
•This protocol describes a method for obtaining quantitative data on the antifungal activity of peptides and other compounds, such as small-molecule antifungal agents, against Candida albicans. Its use of optical density rather than counting colony-forming units to quantify growth inhibition saves time and resources.
Tags
Related Videos
Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides
Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
Quantifying Agonist Activity at G Protein-coupled Receptors
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Assaying the Kinase Activity of LRRK2 in vitro
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
Measurement of Larval Activity in the Drosophila Activity Monitor
Measuring Lactase Enzymatic Activity in the Teaching Lab
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved