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1.7K Views
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05:27 min
September 28th, 2022
DOI :
10.3791/64420-v
Chapters
0:04
Introduction
0:43
Cochlea Removal and Tissue Processing
2:39
Imaging of Cochleae
3:41
Results: Imaging of Cochlea and Analysis of SGN Density Along the Rosenthal s Canal
4:46
Conclusion
Transcript
我们开发了一种光片显微镜,可以将整个耳蜗数字化。该显微镜具有长工作距离的气管物镜,可以对小型小鼠耳蜗到大型人体颞骨进行成像。它用激光束连续切片耳蜗,而不会造成任何机械破坏。
我们的成像方法可用于评估耳蜗的病理变化,例如由于衰老而导致的毛细胞和螺旋神经节神经元的损失。对小鼠实施安乐死并斩首后,通过大脑做一个背腹切口以半切头骨。切除大脑并识别颅骨基腹部分的圆形大疱。
用喇叭打开大疱。然后,可视化并去除耳蜗。在五倍放大的解剖显微镜下,刺穿椭圆形窗口并用锋利的镐取出镫骨。<
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Summary
开发了一种光片显微镜来对整个耳蜗进行成像和数字化。
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