Name: o-ring aortic banding versus traditional transverse aortic constriction for modeling pressure overload-induced cardiac hypertrophy
Uploaded: 2022-10-06T14:00:00
Description: Watch this Scientific Journal Video about O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy at JoVE.com

这项工作得到了联邦电影和研究部（BMBF）对L.L.、N.F.和O.J.M.（IVOLADMT-HF;FKZ 01KC2006A）。

动物实验是在地区委员会的原则下进行的（石勒苏益格-荷尔斯泰因州能源部，国家能源协会，世界，自然和数字化，许可号：V242-21249/2020 [38-4/20]）。用于本研究的小鼠是从商业来源获得的（见 材料表）。将动物保持在标准条件下，光照12小时，夜间周期12小时;随意 提供水和食物。
1. 动物护理
<ol><li>将老鼠关在专门的笼子里，里面有床上用?...

<ol>
	<li>Frey, N., Olson, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+hypertrophy:+The+good,+the+bad,+and+the+ugly.">Cardiac hypertrophy: The good, the bad, and the ugly.</a> Annual Review of Physiology. 65, 45-79 (2003).</li><li>Bui, A. L., Horwich, T. B., Fonarow, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epidemiology+and+risk+profile+of+heart+failure.">Epidemiology and risk profile of heart failure.</a> Nature Reviews Cardiology. 8 (1), 30-41 (2011).</li><li>Bolli, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+initiatives+to+improve+the+rigor+and+reproducibility+of+articles+published+in+Circulation+Research.">New initiatives to improve the rigor and reproducibility of articles published in Circulation Research.</a> Circulation Research. 121 (5), 472-479 (2017).</li><li>Dunlay, S. M., Weston, S. A., Jacobsen, S. J., Roger, V. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Risk+factors+for+heart+failure:+A+population-based+case-control+study.">Risk factors for heart failure: A population-based case-control study.</a> The American Journal of Medicine. 122 (11), 1023-1028 (2009).</li><li>Riehle, C., Bauersachs, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+animal+models+of+heart+failure.">Small animal models of heart failure.</a> Circulation Research. 115 (13), 1838-1849 (2019).</li><li>Rockman, H. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Segregation+of+atrial-specific+and+inducible+expression+of+an+atrial+natriuretic+factor+transgene+in+an+in+vivo+murine+model+of+cardiac+hypertrophy.">Segregation of atrial-specific and inducible expression of an atrial natriuretic factor transgene in an in vivo murine model of cardiac hypertrophy.</a> Proceedings of the National Academy of Sciences of the United States of America. 88 (18), 8277-8281 (1991).</li><li>Hu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Minimally+invasive+aortic+banding+in+mice:+effects+of+altered+cardiomyocyte+insulin+signaling+during+pressure+overload.">Minimally invasive aortic banding in mice: effects of altered cardiomyocyte insulin signaling during pressure overload.</a> American Journal of Physiology-Heart and Circulatory Physiology. 285 (3), 1261-1269 (2003).</li><li>Mohammed, S. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Variable+phenotype+in+murine+transverse+aortic+constriction.">Variable phenotype in murine transverse aortic constriction.</a> Cardiovascular Pathology. 21 (3), 188-198 (2012).</li><li>Nakao, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=O-ring-induced+transverse+aortic+constriction+(OTAC)+is+a+new+simple+method+to+develop+cardiac+hypertrophy+and+heart+failure+in+mice.">O-ring-induced transverse aortic constriction (OTAC) is a new simple method to develop cardiac hypertrophy and heart failure in mice.</a> Scientific Reports. 12, 85 (2022).</li><li>Melleby, A. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+method+for+high+precision+aortic+constriction+that+allows+for+generation+of+specific+cardiac+phenotypes+in+mice.">A novel method for high precision aortic constriction that allows for generation of specific cardiac phenotypes in mice.</a> Cardiovascular Research. 114 (12), 1680-1690 (2018).</li><li>Lindsey, M. L., Kassiri, Z., Virag, J. A. I., de Castro Bras, L. E., Scherrer-Crosbie, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guidelines+for+measuring+cardiac+physiology+in+mice.">Guidelines for measuring cardiac physiology in mice.</a> American Journal of Physiology-Heart and Circulatory Physiology. 314 (4), 733-752 (2018).</li><li>Garcia-Menendez, L., Karamanlidis, G., Kolwicz, S., Tian, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Substrain+specific+response+to+cardiac+pressure+overload+in+C57BL/6+mice.">Substrain specific response to cardiac pressure overload in C57BL/6 mice.</a> American Journal of Physiology-Heart and Circulatory Physiology. 305 (3), 397-402 (2013).</li><li>deAlmeida, A. C., van Oort, R. J., Wehrens, X. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transverse+aortic+constriction+in+mice.">Transverse aortic constriction in mice.</a> Journal of Visualized Experiments. (38), e1729 (2010).</li></ol>

基于线/缝合的主动脉束带已被用于诱导小鼠压力超负荷的心脏肥大。这是一种研究体内心脏重塑和疾病进展的病理机制的既定方法 。 局限性在于狭窄程度的差异相对较高，因此重塑也是如此。最近引入的ORAB技术首先由Melleby等人描述10 通过使用橡胶O形圈优化了传统方法。
该技术最有价值的好处是O形圈的固定直径，这导致狭窄程度的变化较小，因此?...

虽然在发育、运动和怀孕期间可以观察到生理性心脏肥大，但病理性心脏肥大对动脉高血压、瓣膜性心脏病或基因突变等血流动力学应激状况有反应。最初，心脏经历重塑，其特征是心肌细胞大小增加和心室壁增厚以维持心脏功能1，2。另一方面，病理性心脏重塑与心律失常、猝死和高死亡率的风险增加有关。最后，随着时间的推移，它会导致心室扩张，收缩功能严重下降，并最终进展为心力衰竭（HF），这与高发病率，死亡率和社会成本有关3。因此，迫切需要了解分子背景，以便制定新的治疗策略4。
主动脉束带是一种模拟压力超负荷诱导的小鼠左心室 （LV） 肥大和心力衰竭的模型5。使用这种方法，可以检查 体内压力超负荷诱导的心脏重塑的病理机制。Rockman等人报告了小鼠的第一次主动脉束带手术6。压力超负荷是由主动脉（头臂动脉和左颈总动脉之间）周围基于线缝的结扎引起的。为了形成直径为 0.4 mm 的狭窄，在 27 G 针和主动脉周围放置一条缝合线。结扎后，取下针头6，7。
即使针头直径是固定的，线的松紧度也高度依赖于外科医生，因此会影响心脏肥大的诱导表型。此外，在基于线/缝合的方法中，手术后狭窄直径的程度不同，与死亡率的高差异相关8，9。此外，训练这种方法具有挑战性，特别是在找到拧紧螺纹的正确水平和一致性方面。最后，在训练开始时，由于主动脉或其他组织损伤的破坏，以及幸存动物狭窄程度的高度变化，会发生高术中和术后死亡率。
最近，Melleby等人描述了主动脉束带的优化程序10。他们提出了ORAB（O形环主动脉束带）方法，该方法通过使用固定内径为0.71 mm，0.66 mm和0.61 mm的防滑橡胶O形圈，在狭窄方面差异较小，压力过载水平高度可重复。简而言之，O形圈被切开，放置在上升拱形周围，然后用螺纹再次关闭。使用这些O形圈的其他科学家报告了诱导的心脏肥大9的变异性较小。他们还观察到术中和术后死亡率，以及诱导的肥厚表型9，11的更好的可重复性和更少的方差。本文以分步协议描述了这种独特策略的过程。本报告中分享的专业知识将帮助其他科学家改进他们在这一领域的技术。
为了在6周后诱导心脏肥大导致心力衰竭，建议将12周龄的C57BL / 6N雄性小鼠进行手术。小鼠亚系C57BL / 6N和C57BL / 6J之间的主动脉束带后2周的比较显示C57BL / 6N小鼠的严重心功能障碍和相关死亡率增加。因此，这些更适合心力衰竭模型12。十二周龄的雄性和雌性小鼠具有暴露主动脉和用特殊仪器放置O形圈的最佳尺寸。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1 x long Fixators, 6 cm</td>
			<td></td>
			<td>&#160;18200-01</td>
			<td></td>
		</tr>
		<tr>
			<td>2 x Blunt Retractors, 5 mm wide&#160;</td>
			<td></td>
			<td>18200-11</td>
			<td></td>
		</tr>
		<tr>
			<td>2 x Short Fixators, 3 cm</td>
			<td></td>
			<td>&#160;18200-02</td>
			<td></td>
		</tr>
		<tr>
			<td>2 x fine tip 45&#176; angled forceps&#160;</td>
			<td>FST (fine sience tools)</td>
			<td>11160-10</td>
			<td></td>
		</tr>
		<tr>
			<td>3 x Blunt Retractors, 2.5 mm wide&#160;</td>
			<td></td>
			<td>18200-10</td>
			<td></td>
		</tr>
		<tr>
			<td>3 x Retractor Wires&#160;</td>
			<td></td>
			<td>18200-05</td>
			<td></td>
		</tr>
		<tr>
			<td>4-0 absorbable suture (Vicryl)</td>
			<td>ETHICON</td>
			<td>SABBKLT0</td>
			<td>Used to suture skin</td>
		</tr>
		<tr>
			<td>6-0 suture (Prolene), needle size:13 mm</td>
			<td>ETHICON</td>
			<td>JDP879</td>
			<td>Used to suture chest muscle</td>
		</tr>
		<tr>
			<td>8-0 suture (Prolene), needle size: 6.5 mm</td>
			<td>ETHICON</td>
			<td>RHBECH</td>
			<td>Used to fast the Ring&#160;</td>
		</tr>
		<tr>
			<td>Anesthetizing Box, Small</td>
			<td>Havard apparatus&#160;</td>
			<td>50-0108</td>
			<td></td>
		</tr>
		<tr>
			<td>C57BL/6N mice</td>
			<td>Charles River</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Fluosorber Activated Charcoal Filter Canister</td>
			<td>Havard apparatus&#160;</td>
			<td>34-0415</td>
			<td>Used to induce and maintain anesthesia</td>
		</tr>
		<tr>
			<td>Handmade laryngsopcope&#160;</td>
			<td></td>
			<td></td>
			<td>Intubationshelp</td>
		</tr>
		<tr>
			<td>Harvard Apparatus Anesthetic Vaporizer</td>
			<td>Havard apparatus&#160;</td>
			<td></td>
			<td>Used to induce and maintain anesthesia</td>
		</tr>
		<tr>
			<td>Heating pad + rectal probe (LSI Letica Scientific Instruments:Temperature control unit HB 101/2 )</td>
			<td>Panlab/ Havard apparatus&#160;</td>
			<td></td>
			<td>Used to control and maintain body temperature&#160;</td>
		</tr>
		<tr>
			<td>i.v. cannula blue 22-gauge (Vasofix Braun&#252;le 0.9 x 25 mm)</td>
			<td>B/Braunsharing Expertise&#160;</td>
			<td>4268091B</td>
			<td>intubation&#160;</td>
		</tr>
		<tr>
			<td>isofluran</td>
			<td>Baxter</td>
			<td></td>
			<td>Anesthesia</td>
		</tr>
		<tr>
			<td>Kodan (betadine solution)</td>
			<td>Sch&#252;lke</td>
			<td>20003960-A</td>
			<td>Desinfection&#160;</td>
		</tr>
		<tr>
			<td>ligation aid</td>
			<td>FST (fine sience tools)</td>
			<td>18062-12</td>
			<td>Used to perform liagtion with O-ring&#160;</td>
		</tr>
		<tr>
			<td>Microscope Lighting: Schott VisiLED Set MC1500/S80-55 (+ controller)</td>
			<td>SCHOTT</td>
			<td></td>
			<td>Ligth</td>
		</tr>
		<tr>
			<td>Microscope&#160;camera&#160;(Leica IC80 HD)</td>
			<td>Leica</td>
			<td></td>
			<td>Used for visualiation operating field&#160;</td>
		</tr>
		<tr>
			<td>MiniVac Complete Anesthesia Systems&#160;for small rodents</td>
			<td>Havard apparatus&#160;</td>
			<td>75-0233</td>
			<td>Used to induce and maintain anesthesia</td>
		</tr>
		<tr>
			<td>Mouse Ventilator MiniVent Type 845</td>
			<td>Havard Apparatus&#160;</td>
			<td>73-0044</td>
			<td>Used to ventilation during surgery&#160;</td>
		</tr>
		<tr>
			<td>Needle holder&#160;</td>
			<td>FST (fine sience tools)</td>
			<td>&#160;TE-10804</td>
			<td></td>
		</tr>
		<tr>
			<td>O-ring, non-slip rubber (0.0018 mm x 0.020 mm)</td>
			<td>Apple Rubber Products&#160;</td>
			<td></td>
			<td>Liagtion of the aortic arch</td>
		</tr>
		<tr>
			<td>Scissors</td>
			<td>FST (fine sience tools)</td>
			<td>14040-09</td>
			<td>Used to cut the skin and threads&#160;</td>
		</tr>
		<tr>
			<td>Small Animal Retraction System (Kit for Animals up to 200 g)</td>
			<td>FST (fine sience tools)</td>
			<td>18200-20</td>
			<td></td>
		</tr>
		<tr>
			<td>Small Base Plate, 20 x 30 cm&#160;</td>
			<td></td>
			<td>&#160;18200-03</td>
			<td></td>
		</tr>
		<tr>
			<td>Table intgerated with heating pad + rectal probe + ECG and&#160; transducer tripod</td>
			<td>FujiFilm Visual Sonics Imaging System</td>
			<td></td>
			<td>Echocardiography&#160;</td>
		</tr>
		<tr>
			<td>Temgesic (Buprenorphin)</td>
			<td>Indivior UK Limited</td>
			<td>997.00.00</td>
			<td>Pain pre-medication&#160;</td>
		</tr>
		<tr>
			<td>three-way stop cock (blue)</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Tramal (Tamadol)</td>
			<td>Gr&#252;nental</td>
			<td></td>
			<td>Pain post-medication&#160;</td>
		</tr>
		<tr>
			<td>transducer probe MS400 (Visual Sonics)&#160;</td>
			<td>FujiFilm Visual Sonics Imaging System</td>
			<td></td>
			<td>Echocardiography&#160;</td>
		</tr>
		<tr>
			<td>Ultrasound system with cardioligy package&#160;</td>
			<td>FujiFilm Visual Sonics Imaging System</td>
			<td></td>
			<td>Echocardiography&#160;</td>
		</tr>
		<tr>
			<td>Vannas Spring Scissors - 2.5 mm Cutting Edge</td>
			<td>FST (fine sience tools)</td>
			<td>15000-08</td>
			<td>Used to cut intercostal chest muscle&#160;</td>
		</tr>
		<tr>
			<td>vet ointment&#160;</td>
			<td>Bepanten</td>
			<td></td>
			<td>Used to prevent eyes from drying out</td>
		</tr>
	</tbody>
</table>

o-ring aortic banding versus traditional transverse aortic constriction for modeling pressure overload-induced cardiac hypertrophy

小鼠主动脉束带是心脏压力超负荷诱导的心脏肥大和诱导心力衰竭最常用的实验模型之一。以前使用的技术是基于围绕主动脉弓的螺纹缝合线，绑在钝的 27 G 针上以产生狭窄。这种方法取决于外科医生手动拧紧螺纹，因此会导致直径尺寸的巨大差异。Melleby等人描述的一种新改进的方法承诺手术后差异更小，可重复性更高。新技术O形环主动脉束带（ORAB）使用防滑橡胶环代替带线的缝合线，从而减少了压力过载的变化和心脏肥大的可重复表型。在手术过程中，O形圈放置在头臂动脉和左颈动脉之间。超声心动图可确认成功收缩。1 天后，正确放置环会导致横主动脉在 O 形环诱导的狭窄上的流速增加。2 周后，通过射血分数降低和壁厚增加来证明心脏功能受损。重要的是，除了直径大小的差异较小外，与横主动脉收缩 （TAC） 相比，ORAB 与较低的术中和术后死亡率相关。因此，ORAB代表了一种优于常用TAC手术的方法，导致更可重复的结果，并可能减少所需的动物数量。

通常，主动脉束带类似于人类主动脉瓣狭窄并诱导小鼠心脏肥大。成功的手术的特点是心脏组织重塑反映在心脏肥大和心脏功能下降5，6。
手术后1天直接，通过超声心动图13可以在体内确定横主动脉O形环收缩的影响。通过脉冲波多普勒超声测量的主动脉狭窄的血流速度增加，显示了主动脉瓣狭窄的成功诱导...

Watch this Scientific Journal Video about O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy at JoVE.com

O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy

本协议描述了小鼠主动脉束带诱导压力超负荷心脏肥大的新技术。对于捆扎，使用具有固定内径的橡胶圈。这项新技术有望为未来的实验提供更少的方差和更可重复的数据。

O形环主动脉束带与传统横主动脉收缩在压力超负荷引起的心脏肥大建模中的比较

Aortic banding in mice is one of the most commonly used experimental models for cardiac pressure overload-induced cardiac hypertrophy and the induction of ...

这种新的主动脉束带技术允许诱导小鼠压力超负荷心脏肥大，方差较小，重现性更高。在主动脉周围放置一个固定直径的橡胶圈可诱导横主动脉的流速持续增加，从而产生可重复的心脏肥大。该程序应由已经具有啮齿动物麻醉和显微外科技术经验的人执行，或者有经验的人可以指导。首先使用剪刀或手术刀在显微镜下对O形圈进行一次切割，以便在主动脉周围放置。用与 8-0 连接的针刺穿靠近切口的每个环面不可吸收缝合，并拉线。切开，一侧留两到三厘米，另一侧留两厘米，以在最后一步将 O 形环固定在主动脉周围。手术前，取结扎辅助器，并将一个环侧的线端拉过结扎辅助器的孔。将带有附加 O 形圈的结扎助剂放在一边放置。剃除麻醉小鼠左侧胸部的皮毛。然后将小鼠放回充满异氟烷的腔室中，以便在插管前充分镇静。手术前打开加热垫，将动物的体温保持在 37 摄氏度。将加热垫连接到直肠探头以自动保持小鼠的体温。在加热垫周围拉伸橡皮筋，将鼠标与门齿固定在板上。将麻醉的鼠标仰卧放在加热垫上。将橡皮筋放在动物的门牙上，以将颈部伸展到盘子上。将光源聚焦在喉咙上，以便气管插管时气管开口清晰可见。用一只手轻轻张开嘴，另一只手和小镊子放置插管辅助装置。轻轻移动舌头以清除气管开口。将气管插管引入气管，另一方面，继续握住插管辅助装置。通过三向旋塞阀将气管插管位置连接到小鼠呼吸机，以确认正确的气管插管位置。根据制造商的说明监控正确的通风。通过脚趾捏反射检查确认足够的麻醉深度。在眼睛上涂抹眼药膏，以避免手术过程中干燥。使用市售的消毒剂溶液对手术区域进行消毒。使用剪刀，在剑突和左腋窝之间的一条线中间做一个 0.5 到 1 厘米长的皮肤切口。使用镊子将肌肉层与下面的肋骨分开，并将两个五毫米的牵开器放入切口以暴露胸腔。要开始左胸切开术，请使用微型弹簧剪刀在第二和第三肋骨之间的肋间肌肉上进行一到两毫米的切口。打开胸腔，用45度角镊子张开切口。将三个胸部牵开器放入切口以打开胸腔以改善可视化。要暴露主动脉弓，请尝试用细尖 45 度角镊子抬起胸腺和脂肪组织并轻轻地将胸腺和脂肪组织与主动脉弓分开。用一只手用 45 度角的镊子露出主动脉弓。另一方面，定位通过先前连接的一侧螺纹与连接助剂连接的 O 形圈。使用主动脉弓下的结扎辅助工具将线从尾部传递到头臂动脉和左颈总动脉之间的横主动脉的颅侧。用镊子小心地将结扎辅助剂和主动脉弓之间的两根线都取走。取下结扎辅助装置，通过拉动两侧的螺纹，将 O 形圈轻轻地定位在足弓周围。成功定位后，用线和手术结固定 O 形圈。打一个额外的结，以避免在每一侧打开它。结扎O形圈后，从切口中取出三个胸部牵开器。如果需要，通过用温热的 0.9% 等渗盐溶液填充胸部来消除胸部残留的空气。要露出胸廓切口进行缝合，请再次取两个五毫米牵开器以将皮肤保持在侧面。用两条或三条6-0不可吸收的缝合线闭合胸部。取下两个牵开器，用三到五条4-0可吸收缝合线闭合皮肤。然后关闭异氟醚并监测。捏住呼吸机的流出物两秒钟以重新充气肺部。手术后一天，通过测量狭窄处的最大流速，使用超声验证狭窄。对于测量，请使用超声心动图和超声系统和频率为 30 兆赫兹的换能器探头。将麻醉动物仰卧放在加热垫上，并将加热垫与直肠探头连接以将体温保持在37摄氏度，并使用四个鼠标爪传感器用心电图监测心率。通过超声测量，成功实施 O 形环主动脉束带可增加狭窄处的流速。对于此测量，将换能器头放在右侧胸骨旁，以通过二维成像定位主动脉弓。使用彩色多普勒观察主动脉中的血流，并用脉冲波多普勒测量狭窄处的血流速度。通过二维成像可视化左右颈总动脉。将换能器头水平放置在颈部的左右两侧，呈 45 度角，并使用脉冲波多普勒确定血流速度。通过脉冲波多普勒超声测量的主动脉狭窄的血流速度增加，成功诱导主动脉瓣狭窄。此外，左右颈总动脉之间的血流速度比被确定为O形环主动脉束带的功能标志物。术后两周，观察到舒张期左心室后壁和脑室间隔直径增加。此外，通过测量左心室射血分数降低，记录了心脏功能的进行性恶化。在将 O 形环固定在主动脉弓周围时，重要的是要用结关闭两侧的环以获得最佳效果。

Research

JoVE Journal

Medicine

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

科研

医学

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

在横向主动脉缩窄的小鼠模型和心脏形态学评估功能改变通过超声心动图成像

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Minimally Invasive Transverse Aortic Constriction in Mice

微创横向主动脉缩窄小鼠

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots

全根主动脉瓣置换主动脉瓣狭窄主动脉异种移植患者小主动脉根

In patients with small aortic roots who need an aortic valve replacement with biological valve substitutes, the implantation of the stented pericardial ...

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

小鼠横主动脉狭窄诱导左心室肥厚的技术研究

Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure ...

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

主动脉瓣重置术治疗瓣膜备用主动脉根置换的规范化技术

Despite the obvious advantages of the preservation of a normal aortic valve during aortic root replacement, the complexity of valve sparing procedures ...

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice

诱导小鼠横向主动脉狭窄的闭合胸模型

Research on cardiac hypertrophy and heart failure is frequently based on pressure overload mouse models induced by TAC. The standard procedure is to ...

A Pulmonary Trunk Banding Model of Pressure Overload Induced Right Ventricular Hypertrophy and Failure

压力超载诱发右室肥厚和衰竭的肺动脉扣条柱模型

Right ventricular (RV) failure induced by sustained pressure overload is a major contributor to morbidity and mortality in several cardiopulmonary ...

Three-Dimensional Printing of a Complex Aortic Anomaly

复杂主动脉异常的三维打印

Complex congenital aortic anomalies include diverse types of malformations that may be clinically asymptomatic or present with respiratory or esophageal ...