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Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria

1.7K Views

10:35 min

October 6th, 2022

October 6th, 2022

1,728 Views

0:04

Introduction

0:57

Crude Plastoglobule Extraction from Un-Stressed Maize Leaf Tissue

4:41

Crude Plastoglobule Extraction from Cyanobacteria

7:06

Harvesting Pure Plastoglobules

7:55

Cyanobacteria Processing

8:43

Results: Isolation of Plastoglobule Lipid Droplets Associated with Various Photosynthetic Organisms

9:36

Conclusion

Transcript

The isolation of highly pure plastoglobules is critical to their study. A rapid and effective protocol for their isolation that facilitates their downstream biochemical investigation is presented here. The primary benefit of this method is the rel

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A fast and efficient protocol is presented for the isolation of plastoglobule lipid droplets associated with various photosynthetic organisms. The successful preparation of isolated plastoglobules is a crucial first step that precedes detailed molecular investigations such as proteomic and lipidomic analyses.

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