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1.5K Views
•
09:33 min
January 6th, 2023
DOI :
10.3791/64519-v
Chapters
0:04
Introduction
0:20
Preparation of the Silicone Grafting Device
2:01
Preparation of Seedlings for Grafting
4:39
Procedure for Grafting
7:25
Results: Grafting Trial Variables and Success Rates
9:06
Conclusion
Transcript
早期播种嫁接是在植物中设置长距离信号的有力工具。我们希望这种方法将有助于成功采用播种嫁接,而无需动手培训。首先准备方形培养皿,尺寸为100x100毫米,用于浇注有机硅弹性体。
用丙酮去除电线上的外层纸涂层后,取 29 号线扎带。并通过在坚硬、均匀的表面上滚动金属丝来完全拉直电线,并带有沉重的扁平物体,例如金属管架。然后,在方形培养皿中等距放置四根拉直的电线,确保电线平放在培养皿底部。
将混合硅弹性体溶液倒在培养皿中的电线顶部,然后将盖子放在培养皿上。让硅胶在室温下固
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Summary
该协议描述了一种强大的幼苗嫁接方法,该方法不需要先前的经验或培训,并且可以使用大多数分子生物学实验室中易于获得的材料以非常低的成本执行。
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