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JoVE Journal

Biochemistry

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

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07:03 min

December 23rd, 2022

December 23rd, 2022

2,516 Views

0:04

Introduction

0:56

Co-Transformation of Escherichia coli

2:18

Expression

3:21

Pulldown Assay

4:52

Results: Bacterial Co-Expression of Differentially Tagged Proteins

6:15

Conclusion

Transcript

This method allows to study formations of protein complexes that require co-expression for efficient test samples, such as the formation of heterodimers. Main end potential of this method is exploiting the co-expression of differentially-tagged st

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Here, we describe a method for the bacterial co-expression of differentially tagged proteins using a set of compatible vectors, followed by the conventional pulldown techniques to study protein complexes that cannot assemble in vitro.

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