Determining the Rickettsia parkeri Infection Rate via Geimsa Staining
1:35
Purification of Rickettsia parkeri from Tick Cell Culture
3:35
Transformation of Rickettsia parkeri with the pRAM18dSFA Plasmid
5:52
Results: Transformants of Rickettsia parkeri Expressing RFP in ISE6 Cells
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Conclusion
Transcript
Functional studies of Rickettsia genes are crucial for understanding their role in pathogenesis, so we need dependable methods of altering the genes in a controlled fashion. Our protocol uses electroporation to introduce exogenous DNA into obligat
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Electroporation is a rapid, broadly adopted method for introducing exogenous DNA into the genus Rickettsia. This protocol provides a useful electroporation method for the transformation of obligate intracellular bacteria in the genus Rickettsia.