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06:37 min
December 23rd, 2022
DOI :
10.3791/64573-v
Chapters
0:04
Introduction
0:29
Isolation of T Cells with Microbubbles Using Positive Selection
1:51
Co-Stimulation (Activation) of the Positively Selected T Cells
2:52
Expansion of the Co-Stimulated Cells in Cell Culture Medium
3:28
Expansion of the T Cells (With or Without Prior Transduction)
4:02
Harvesting the T Cells and Flow Cytometry
5:16
Results: Assessment of Viable and Successfully Transduced Cells
5:58
Conclusion
Transcript
微泡活化和扩增方案意义重大,因为它解决了细胞疗法研究和制造中未满足的主要需求,提高了T细胞疗法的持久性和疗效。Akadeum基于浮力的正选择、激活和扩增技术限制了激活和扩增工作流程期间的过度刺激和随后的T细胞衰竭。首先,用生物素化的抗CD3抗体在2.5毫升分离缓冲液中孵育3次10至第8个商业获得的PBMC。
通过移液轻轻混合组分,并在室温下孵育10分钟。根据制造商的说明,以0.5:1的比例将剥离的亲和素微泡添加到细胞中。并使用商用端到端旋转器在室温下以 20 RPM 混合 10
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Summary
本研究的目的是证明基于浮选的分离在分离、激活和扩增原代人T细胞方面的可行性。
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