Isolation of T Cells with Microbubbles Using Positive Selection
1:51
Co-Stimulation (Activation) of the Positively Selected T Cells
2:52
Expansion of the Co-Stimulated Cells in Cell Culture Medium
3:28
Expansion of the T Cells (With or Without Prior Transduction)
4:02
Harvesting the T Cells and Flow Cytometry
5:16
Results: Assessment of Viable and Successfully Transduced Cells
5:58
Conclusion
Transcript
Mikrobobleaktiverings- og ekspansionsprotokollen er vigtig, fordi den adresserer et stort uopfyldt behov inden for celleterapiforskning og fremstilling, hvilket forbedrer persistensen og effektiviteten af T-celleterapier. Akadeums opdriftsbaserede
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Formålet med denne undersøgelse er at demonstrere muligheden for flotationsbaseret adskillelse for at isolere, aktivere og udvide primære humane T-celler.