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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

DOI :

10.3791/64574-v

12:14 min

September 14th, 2022

September 14th, 2022

3,334 Views

1Department of Neurology, Yale University, 2Department of Neuroscience, Yale University, 3Interdepartmental Neuroscience Program, Yale University

This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

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Subcellular Fractionation

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