Name: enrichment of adult mouse dorsal root ganglia neuron cultures by immunopanning
Uploaded: 2023-02-24T14:15:00
Description: Watch this Scientific Journal Video about Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning at JoVE.com

这项工作得到了加拿大卫生研究院的项目资助（FRN-162434）和加拿大MS协会的发现资助（EGID-3761）。作者要感谢孙博士和交叉癌症研究所对ImageXpress系统的培训和使用。

所有动物实验均经阿尔伯塔大学健康科学动物护理和使用委员会（协议0000274）批准进行。
1. 试剂制备
<ol><li>对于第1天，准备以下试剂：细胞培养级水;聚-D-赖氨酸-通过将整个瓶子重新配制在50 mL培养级水中至100 μg/mL储备液浓度来制备储备液，储存在4 °C，并将储备液在培养级水中以1：10稀释至终浓度为10 μg/ mL;tris-HCl-通过将盐酸三盐酸粉末溶解在培养级水中至50...

<ol>
	<li>Haberberger, R. V., Barry, C., Dominguez, N., Matusica, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+dorsal+root+ganglia.">Human dorsal root ganglia.</a> Frontiers in Cellular Neuroscience. 13, 271 (2019).</li><li>Perner, C., Sokol, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protocol+for+dissection+and+culture+of+murine+dorsal+root+ganglia+neurons+to+study+neuropeptide+release.">Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release.</a> STAR Protocols. 2 (1), 100333 (2021).</li><li>Lin, Y. T., Chen, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dorsal+root+ganglia+isolation+and+primary+culture+to+study+neurotransmitter+release.">Dorsal root ganglia isolation and primary culture to study neurotransmitter release.</a> Journal of Visualized Experiments:JoVE. (140), e57569 (2018).</li><li>Foo, L. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+method+for+the+purification+and+culture+of+rodent+astrocytes.">Development of a method for the purification and culture of rodent astrocytes.</a> Neuron. 71 (5), 799-811 (2011).</li><li>Nolle, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enrichment+of+Glial+Cells+From+Human+Post-mortem+Tissue+for+Transcriptome+and+Proteome+Analysis+Using+Immunopanning.">Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning.</a> Frontiers in Cellular Neuroscience. 15, 772011 (2021).</li><li>Barres, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Designing+and+troubleshooting+immunopanning+protocols+for+purifying+neural+cells.">Designing and troubleshooting immunopanning protocols for purifying neural cells.</a> Cold Spring Harbor Protocols. 2014 (12), 1342-1347 (2014).</li><li>Zuchero, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Purification+of+dorsal+root+ganglion+neurons+from+rat+by+immunopanning.">Purification of dorsal root ganglion neurons from rat by immunopanning.</a> Cold Spring Harbor Protocols. 2014 (8), 826-838 (2014).</li><li>Sommer, I., Schachner, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monoclonal+antibodies+(O1+to+O4)+to+oligodendrocyte+cell+surfaces:+an+immunocytological+study+in+the+central+nervous+system.">Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system.</a> Developmental Biology. 83 (2), 311-327 (1981).</li><li>Sommer, I., Schachner, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+that+are+O4+antigen-positive+and+O1+antigen-negative+differentiate+into+O1+antigen-positive+oligodendrocytes.">Cell that are O4 antigen-positive and O1 antigen-negative differentiate into O1 antigen-positive oligodendrocytes.</a> Neuroscience Letters. 29 (2), 183-188 (1982).</li><li>Hanani, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Satellite+glial+cells+in+sensory+ganglia:+from+form+to+function.">Satellite glial cells in sensory ganglia: from form to function.</a> Brain Research. Brain Research Reviews. 48 (3), 457-476 (2005).</li><li>Viveiros, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In-cell+labeling+coupled+to+direct+analysis+of+extracellular+vesicles+in+the+conditioned+medium+to+study+extracellular+vesicles+secretion+with+minimum+sample+processing+and+particle+loss.">In-cell labeling coupled to direct analysis of extracellular vesicles in the conditioned medium to study extracellular vesicles secretion with minimum sample processing and particle loss.</a> Cells. 11 (3), 351 (2022).</li></ol>

这种免疫平移方案增加了DRG原代培养物中神经元细胞的比例。为了获得最佳效果，应及时进行解剖，并应修剪多余的神经。解离步骤应仔细监测，不应超过1小时，以防止细胞受到不必要的压力。特别是关于免疫淘选，每个板应在中点轻轻旋转，以使细胞能够接触到包被的抗体。收集神经元细胞悬液后，还应在培养显微镜下观察平板，以确保非神经元细胞已结合。
使用成年小?...

背根神经节（DRGs）容纳支配外周组织的感觉神经元的细胞体。研究这些神经元使我们能够理解疼痛和感觉状况的机制基础。然而，DRGs不仅由神经元组成，还包含成纤维细胞，雪旺细胞，巨噬细胞和其他免疫细胞1。尽管存在这些不同的细胞类型，但整个DRG培养物在文献中通常被称为神经元2，3。这些培养物仍然可用于通过成像或流式细胞术进行神经元研究，这将允许通过染色、细胞大小和/或形态进行神经元鉴定。然而，对于聚合酶链反应（PCR）或蛋白质印迹等检测，其中培养物均质化以进行RNA或蛋白质收集，非神经元细胞类型的存在可能会干扰结果。因此，需要增加DRG培养物中神经元细胞的比例。
免疫淘选是一种用于纯化各种细胞类型的技术，包括皮质神经元、星形胶质细胞、少突胶质细胞前体细胞和小胶质细胞。简而言之，它涉及将针对细胞表面标志物的抗体粘附到培养皿上，旨在结合某些细胞（图1），并且可用于选择支持或反对感兴趣的细胞类型4，5，6。免疫淘选大鼠胚胎DRG以选择针对非神经元细胞，之前已由Zuchero7描述过。然而，我们一直无法找到特定于小鼠DRG的免疫淘选方案。在该协议中，我们以Zuchero协议的基本租户为基础，但改为调整免疫淘选序列以丰富成年小鼠DRG培养物（图2）。这是研究培养中已建立的感觉神经元的有力工具。它是有利的，因为它允许使用来自遗传，疾病和损伤模型的成年小鼠，以便可以更特异性地研究它们的感觉神经元。
该协议对于希望增加成年小鼠DRG培养物中神经元比例的读者是有利的。然而，免疫淘选步骤也可以省略，该方案也可用于整个DRG培养。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>0.2 um Syringe filters</td>
			<td>Fisher</td>
			<td>723-2520</td>
			<td></td>
		</tr>
		<tr>
			<td>100 mm petri dishes</td>
			<td>ThermoFisher</td>
			<td>FB0875712</td>
			<td></td>
		</tr>
		<tr>
			<td>24 well black glass-bottom plates</td>
			<td>Cellvis</td>
			<td>P24-1.5H-N</td>
			<td></td>
		</tr>
		<tr>
			<td>70 um cell strainer</td>
			<td>Cedarlane</td>
			<td>15-1070-1(BI)</td>
			<td></td>
		</tr>
		<tr>
			<td>B27+ supplement</td>
			<td>Gibco</td>
			<td>A3582801</td>
			<td></td>
		</tr>
		<tr>
			<td>Bovine Serum Albumin</td>
			<td>Sigma Aldrich</td>
			<td>A7906</td>
			<td>for 15% BSA cushion, and ICC (heat shock fraction &#8805;98%)</td>
		</tr>
		<tr>
			<td>Bovine Serum Albumin</td>
			<td>Sigma Aldrich</td>
			<td>A4161</td>
			<td>for 4% BSA for immunopanning, and SATO for media (essentially globulin free, suitable for cell culture, &#8805;99%)</td>
		</tr>
		<tr>
			<td>CD45 antibody</td>
			<td>BD Pharmigen</td>
			<td>550539</td>
			<td></td>
		</tr>
		<tr>
			<td>DAPI</td>
			<td>Invitrogen</td>
			<td>D1306</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM</td>
			<td>Gibco</td>
			<td>11960069</td>
			<td></td>
		</tr>
		<tr>
			<td>DNAse</td>
			<td>Worthington</td>
			<td>LS002007</td>
			<td>for stemxyme solution and panning buffer</td>
		</tr>
		<tr>
			<td>D-PBS</td>
			<td>Sigma Aldrich</td>
			<td>D8662</td>
			<td></td>
		</tr>
		<tr>
			<td>EBSS</td>
			<td>Sigma Aldrich</td>
			<td>E6267</td>
			<td>for DNAse solution</td>
		</tr>
		<tr>
			<td>Filter paper P8 grade</td>
			<td>ThermoFisher</td>
			<td>09-795K</td>
			<td>for 8% PFA</td>
		</tr>
		<tr>
			<td>Glutamax</td>
			<td>ThermoFisher</td>
			<td>35050061</td>
			<td></td>
		</tr>
		<tr>
			<td>goat-anti-mouse IgG</td>
			<td>Jackson Immunoresearch</td>
			<td>115-005-020</td>
			<td>for O4 dish&#160;</td>
		</tr>
		<tr>
			<td>goat-anti-rabbit 488</td>
			<td>Invitrogen</td>
			<td>A11008</td>
			<td></td>
		</tr>
		<tr>
			<td>goat-anti-rabbit IgG</td>
			<td>Jackson Immunoresearch</td>
			<td>115-005-003</td>
			<td>for PDGFRB dish</td>
		</tr>
		<tr>
			<td>goat-anti-rat IgG</td>
			<td>Jackson Immunoresearch</td>
			<td>115-005-044</td>
			<td>for CD45 dish</td>
		</tr>
		<tr>
			<td>HBSS -/-</td>
			<td>Sigma Aldrich</td>
			<td>14175145</td>
			<td></td>
		</tr>
		<tr>
			<td>Insulin</td>
			<td>Sigma Aldrich</td>
			<td>I2643</td>
			<td></td>
		</tr>
		<tr>
			<td>laminin</td>
			<td>Invitrogen</td>
			<td>23017-015</td>
			<td></td>
		</tr>
		<tr>
			<td>N-acetyl cysteine</td>
			<td>Sigma Aldrich</td>
			<td>A8199</td>
			<td></td>
		</tr>
		<tr>
			<td>Neurobasal</td>
			<td>Gibco</td>
			<td>21103049</td>
			<td></td>
		</tr>
		<tr>
			<td>Normal Donkey Serum</td>
			<td>Sigma-Aldrich</td>
			<td>D9663</td>
			<td></td>
		</tr>
		<tr>
			<td>O4 antibody</td>
			<td>n/a</td>
			<td>n/a</td>
			<td>Hybridoma</td>
		</tr>
		<tr>
			<td>Ovomucoid trypsin inhibitor</td>
			<td>Cedarlane</td>
			<td>LS003086</td>
			<td>for low ovo</td>
		</tr>
		<tr>
			<td>paraformaldehyde prills</td>
			<td>Sigma Aldrich</td>
			<td>441244</td>
			<td>for 8% PFA</td>
		</tr>
		<tr>
			<td>PDGFRB antibody</td>
			<td>Abcam</td>
			<td>AB32570</td>
			<td></td>
		</tr>
		<tr>
			<td>penicillin/ streptomycin</td>
			<td>Gibco</td>
			<td>15140-122</td>
			<td></td>
		</tr>
		<tr>
			<td>Poly-D-Lysine</td>
			<td>Sigma Aldrich</td>
			<td>P6407</td>
			<td></td>
		</tr>
		<tr>
			<td>Progesterone</td>
			<td>Sigma Aldrich</td>
			<td>P8783</td>
			<td>for SATO</td>
		</tr>
		<tr>
			<td>Putrescine dihydrochrloride&#160;</td>
			<td>Sigma Aldrich</td>
			<td>P5780</td>
			<td>for SATO</td>
		</tr>
		<tr>
			<td>Sodium phosphate dibasic</td>
			<td>Fisher</td>
			<td>S374-1</td>
			<td>for 0.2 M PB</td>
		</tr>
		<tr>
			<td>Sodium Phosphate monobasic dihydrate</td>
			<td>Sigma Aldrich</td>
			<td>04269</td>
			<td>for 0.2 M PB</td>
		</tr>
		<tr>
			<td>Sodium Pyruvate</td>
			<td>ThermoFisher</td>
			<td>11360070</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium Selenite</td>
			<td>Sigma Aldrich</td>
			<td>S5261</td>
			<td>for SATO</td>
		</tr>
		<tr>
			<td>Stemxyme I</td>
			<td>Cedarlane</td>
			<td>LS004107</td>
			<td>for tissue dissociation; combination collagenase</td>
		</tr>
		<tr>
			<td>Transferrin</td>
			<td>Sigma Aldrich</td>
			<td>T1147</td>
			<td>for SATO</td>
		</tr>
		<tr>
			<td>Tris-HCl</td>
			<td>Millipore Sigma</td>
			<td>T5941</td>
			<td></td>
		</tr>
		<tr>
			<td>trypan blue</td>
			<td>Gibco</td>
			<td>15250-061</td>
			<td></td>
		</tr>
		<tr>
			<td>&#946;3-Tubulin</td>
			<td>Sigma-Aldrich</td>
			<td>T2200</td>
			<td></td>
		</tr>
	</tbody>
</table>

enrichment of adult mouse dorsal root ganglia neuron cultures by immunopanning

背根神经节（DRGs）是与脊髓背角相邻的外围结构，容纳感觉神经元的细胞体以及各种其他细胞类型。已发表的培养方案通常将整个解离的DRG培养物称为神经元，尽管存在成纤维细胞，雪旺细胞，巨噬细胞和淋巴细胞。虽然这些完整的DRG培养物足以用于成像应用，其中可以根据形态或染色来识别神经元，但从这些培养物中收集的蛋白质或RNA匀浆主要不是神经元起源的。在这里，我们描述了培养小鼠DRG的免疫平移序列。该方法的目标是通过去除其他细胞类型来丰富神经元的DRG培养物。免疫淘选是指通过将抗体粘附到细胞培养皿中来去除细胞类型的方法。使用这些培养皿，我们可以消极地选择和减少培养物中成纤维细胞、免疫细胞和雪旺细胞的数量。这种方法使我们能够增加培养物中神经元的百分比。

然后用共聚焦高内涵筛选系统对用DAPI和β3-微管蛋白染色的固定细胞进行成像。使用合适的商业软件分析图像，以确定与β3-微管蛋白共标记的DAPI阳性细胞的百分比（图3）。确定整个DRG培养物具有42.36%±6.4%β3-微管蛋白染色，免疫泛洗DRG培养物具有71.44%±7.43%β3-微管蛋白染色。这表明免疫淘移的神经元富集显着增加（p < 0.0001，未配对t检验）。
<p class="jove_content biglegen...

Watch this Scientific Journal Video about Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning at JoVE.com

Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning

本文描述了一种成年小鼠背根神经节的免疫平移方案。通过将抗体粘附到培养板上，我们可以负性地选择和去除非神经元细胞。我们表明，使用该协议为神经元富集培养物，从而可以深入研究神经元对操作的反应。

通过免疫淘选富集成年小鼠背根神经节神经元培养物

Dorsal root ganglia (DRGs) are peripheral structures adjacent to the dorsal horn of the spinal cord, which house the cell bodies of sensory neurons as ...

该协议使我们能够增强成年小鼠背根神经节培养物中的神经元数量。这可能有助于确定神经元细胞对给定反应的贡献。我们在基本的DRG培养方案中增加了一个免疫淘选步骤。主要优点是选择针对非神经元细胞。如果您不熟悉原发性 DRG 培养，最好练习解剖以获得尽可能多的 DRG，并尽可能多地修剪神经。首先，吸出用于包被培养板的聚-D-赖氨酸，并用组织培养水洗涤板三次。倾斜盖子，让表面完全干燥。将足够的层粘连蛋白涂在板上以涂覆每个孔的底部，并将其置于37摄氏度的培养箱中。用D-PBS清洗先前准备好的CD45，PDGFR-β和O4淘洗盘。对于CD45培养皿，加入5毫升0.2%BSA和20微升CD45一抗。对于 PDGFR-β 培养皿，将 15.2 微升 PDGFR-β 添加到 5 毫升含 0.2% BSA 的平板中。对于 O4 培养皿，将 2 毫升 O4 杂交瘤加入 3 毫升 0.2% BSA 和另外 100 微升 4%BSA，以确保最终 BSA 浓度保持在 0.2%接下来，用 30 毫升 0.9% 冰冷盐水灌注安乐死小鼠。观察肝脏颜色的变化以确保灌注。将前爪和后爪别在泡沫塑料平台上，并使用干净的剃须刀片从背部露出脊柱。通过在脊柱背侧切开大约一半深的切口，切除脊柱的背半部分以露出脊髓来进行椎板切除术。要么轻轻切断神经并移除脊髓，要么轻轻地将脊髓推到一边，保持神经完整性。在解剖显微镜下，使用脊神经根从脊柱两侧找到并去除所有背根神经节或DRG。去除每个DRG的神经碎片时，修剪其，因为培养物中更多的神经碎片可能导致生存率低下。将DRG收集在冰上的锥形管中，将15毫升HBSS中的DRG，并让它们沉降到底部。使用移液器从DRG中吸出大部分HBSS，并在管中留下约100微升液体，以避免丢失组织。用1毫升HBSS洗涤三次，并向组织中加入5毫升先前解冻的工作STEMxyme溶液。用透明薄膜盖住盖子，然后将管子侧浮在 37 摄氏度的水浴中一小时。与酶孵育后，向管中加入1毫升低卵木样抑制剂溶液。用P1000移液器轻轻研磨细胞10至15次，并让组织块沉降。然后将含有解离细胞的顶部2至3毫升溶液转移到新鲜的低卵粘蛋白溶液中，并重复直到组织完全解离，并且没有可见块残留。在室温下以300×g离心管10分钟。如前所述，使用移液管除去上清液后，将细胞沉淀重悬于1毫升淘洗缓冲液中，然后轻轻移液混合。在50毫升锥形管上用1毫升淘洗缓冲液预润湿70微米细胞过滤器，然后通过细胞过滤器过滤细胞溶液。用1毫升淘洗缓冲液清洗试管，然后将其通过过滤器。对于细胞分层，加入2毫升15%BSA，并轻轻涂覆封闭管的侧面。为了去除髓磷脂碎片，通过将移液器靠在管的侧面，在BSA垫子的顶部，一次小心地将细胞悬液分层一毫升。在室温下以300×g离心管10分钟，缓慢加速和减速。中间髓鞘层显示白色物质片。使用 P1000 移液器，一次取出一毫升，去除顶部的透明液体、中间的髓磷脂相和 BSA，留下约 100 微升以免干扰沉淀。对于抗原修复，加入 5 毫升淘洗缓冲液并将试管在 37 摄氏度和 10% 二氧化碳培养箱中孵育 30 至 45 分钟。接下来，用D-PBS清洗孵育的CD45培养皿三次，然后倒出最后的冲洗液。然后将细胞悬液倒入CD45培养皿中。将培养皿在室温下孵育20分钟，每隔10分钟轻轻旋转一次，以使细胞平等地获得抗体。轻轻摇晃 CD45 培养皿并以一定角度支撑它。小心地将1毫升细胞悬液移过培养皿上，以收集未结合的细胞。然后将其转移到PDGFR-β培养皿中，先前用D-PBS洗涤三次，并孵育。如前所述，将未结合的细胞从PDGFR-β培养皿转移到O 4培养皿中，并孵育板。孵育后，将细胞悬液转移到15毫升锥形管中，并以300×g离心10分钟。将细胞重悬至所需体积，并用台盼蓝稀释至1：1的比例以获得细胞活力。使用血细胞计数器，计数中到大细胞。取出层粘连蛋白并立即以所需密度接种细胞，在孵育板之前不让它们干燥。固定、完整和免疫泛选的DRG培养物用β3-微管蛋白染色神经元，DAPI染色所有细胞核。确定整个DRG培养物具有约42.36%的β3-微管蛋白染色，免疫泛选的DRG培养物具有约71.44%的β3-微管蛋白染色，这表明免疫淘选的神经元富集显着增加。小心治疗组织非常重要。轻柔地处理DRG，修剪多余的神经，注意不要搅动或失去组织和培养物，这些都是需要注意的关键。

Research

JoVE Journal

Neuroscience

Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury

染色质免疫沉淀从以下轴索损伤的背根神经节组织

Axons in the central nervous system (CNS) do not regenerate while those in the peripheral nervous system (PNS) do regenerate 
to a limited extent after ...

科研

神经科学

Live Imaging of Dorsal Root Axons after Rhizotomy

实时成像背根轴突后根切断术

The primary sensory axons injured by spinal root injuries fail to regenerate into the spinal cord, leading to chronic pain and permanent sensory loss. ...

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

培养成年的背根神经节神经元轴突再生的遗传研究

It is well known that mature neurons in the central nervous system (CNS) cannot regenerate their axons after injuries due to diminished intrinsic ability ...

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo

测量脊髓突触前抑制小鼠背根电位记录<em&gt;在体内</em

Presynaptic inhibition is one of the most powerful inhibitory mechanisms in the spinal cord. The underlying physiological mechanism is a depolarization of ...

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

背根神经节神经元和区分脂肪干细胞：一种<em&gt;在体外</em&gt;共培养模型来研究周围神经再生

Dorsal root ganglia (DRG) neurons, located in the intervertebral foramina of the spinal column, can be used to create an in vitro system facilitating the ...

Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents

背根神经节的啮齿动物的脊髓和隔离的液压挤压

Traditionally, the spinal cord is isolated by laminectomy, i.e. by breaking open the spinal vertebrae one at a time. This is both time consuming and may ...

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

背根神经节内注射和背根挤压伤为感官轴突再生模型

Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each ...

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

背根神经节分离与原代培养对神经递质释放的研究

Dorsal root ganglia (DRG) contain cell bodies of sensory neurons. This type of neuron is pseudo-unipolar, with two axons that innervate peripheral ...

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

哺乳动物轴突再生: 成年小鼠背根神经节体内电穿孔的研究

Electroporation is an essential non-viral gene transfection approach to introduce DNA plasmids or small RNA molecules into cells. A sensory neuron in the ...

Rapid Isolation of Dorsal Root Ganglion Macrophages

多尾根结鱼巨噬细胞的快速隔离

There are growing interests to study the molecular and cellular interactions among immune cells and sensory neurons in the dorsal root ganglia after ...