Denne protokol tillader effektiv og reproducerbar differentiering af brune adipocytter, der udtrykker vigtige adipogene transkriptionsfaktorer, modne adipocytmarkører og har termogent potentiale og morfologisk fænotype af modne brune adipocytter.
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.