Dit protocol maakt de efficiënte en reproduceerbare differentiatie mogelijk van bruine vetocyten die belangrijke adipogene transcriptiefactoren, rijpe adipocytmarkers tot expressie brengen en thermogeen potentieel en morfologisch fenotype van rijp
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.