Dieses Protokoll ermöglicht die effiziente und reproduzierbare Differenzierung von braunen Adipozyten, die wichtige adipogene Transkriptionsfaktoren und reife Adipozytenmarker exprimieren und das thermogene Potenzial und den morphologischen Phänot
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.