Questo protocollo consente la differenziazione efficiente e riproducibile di adipociti bruni che esprimono fattori chiave di trascrizione adipogenica, marcatori adipocitari maturi e hanno un potenziale termogenico e un fenotipo morfologico degli a
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.