Este protocolo permite la diferenciación eficiente y reproducible de adipocitos marrones que expresan factores de transcripción adipogénicos clave, marcadores de adipocitos maduros y tienen potencial termogénico y fenotipo morfológico de adipocito
Sign in or start your free trial to access this content
Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.