Detta protokoll möjliggör effektiv och reproducerbar differentiering av bruna adipocyter som uttrycker viktiga adipogena transkriptionsfaktorer, mogna adipocytmarkörer och har termogen potential och morfologisk fenotyp av mogna bruna adipocyter. D
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.