Bu protokol, temel adipojenik transkripsiyon faktörlerini, olgun adiposit belirteçlerini eksprese eden ve olgun kahverengi adipositlerin termojenik potansiyeline ve morfolojik fenotipine sahip kahverengi adipositlerin verimli ve tekrarlanabilir fa
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.