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A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

DOI :

10.3791/64634-v

December 9th, 2022

December 9th, 2022

2,563 Views

1The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences, Lund University, 2Department of Autoimmunity, BTB Emulsions

To induce experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis, mice are immunized with a water-in-oil emulsion containing an autoantigen and complete Freund's adjuvant. While several protocols exist for the preparation of these emulsions, a rapid, simple, and standardized homogenization protocol for emulsion preparation is presented here.

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Homogenization Method

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