Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
762 Views
•
08:54 min
February 17th, 2023
DOI :
10.3791/64677-v
Chapters
0:04
Introduction
0:37
Preparation of IR783/BC Nanoparticles by the Flash Precipitation Method
2:52
Characterization of IR783/BC Nanoparticles
4:47
Photoactivation of IR783/BC Nanoparticles
6:24
Results: Fabrication and Characterization of a Photoresponsive Prodrug-Dye Nano Assembly
8:27
Conclusion
Transcript
该协议为光响应药物染料系统的构建和表征提供了参考,特别是光辐射的设置。该技术显示出制造简单、载药量高、光控性好等优点。该技术可用于在光纤的帮助下治疗结直肠肿瘤,以传递光以激活肿瘤部位的药物释放。
首先称量10毫克硼二吡咯甲烷苯丁酸氮芥或BC前药,并将其溶解在1.5毫升微管中的一毫升二甲基亚砜或DMSO中。用箔纸盖住BC溶液,然后在过滤的去离子水中制备每毫升0.4毫克的IR783,并在1.5毫升微管中转移300微升。将此微管置于1500 RPM的涡旋混合器上。
接下来
Sign in or start your free trial to access this content
Summary
该协议描述了光响应前药染料纳米组装的制造和表征。明确描述了通过光触发拆卸从纳米颗粒释放药物的方法,包括光照射设置。光照射后从纳米颗粒释放的药物对人结直肠肿瘤细胞表现出优异的抗增殖作用。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved