Results: Analyzing PERIOD-mNeonGreen Protein Expression in Clock Neurons
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Conclusion
Transcript
The purpose of this protocol is to generate an endogenous edited fly line. We show a fluorescent protein knock-in as an example, but it could also be applied to other knock-in, knockout, as well as mutation generation. Our protocol is a CRISPR hom
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Here, we present a simplified endogenous gene tagging protocol for Drosophila, which utilizes a PCR-based technique for marker-free identification of successful genetic modifications, facilitating the development of stable knock-in lines.