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822 Views
•
09:09 min
February 17th, 2023
DOI :
10.3791/64807-v
Chapters
0:05
Introduction
0:54
Thawing of Cryopreserved Ovarian Tissue
1:58
Follicle Isolation
4:26
RNA Extraction
7:01
Results: Isolation and Gene Expression Analyses in Human Ovarian Follicles
8:16
Conclusion
Transcript
该协议描述了一种从周围基质细胞中分离卵巢卵泡以评估这些生殖细胞的基因表达分析的有效方法。这种技术使用受控的酶和机械分离,允许在保持其完整性的同时检索卵泡。按照该协议,研究人员可以评估这些细胞的特定基因表达。
该技术可用于进一步了解与影响卵巢功能的疾病或暴露于有毒物质后相关的卵泡中基因表达的缺陷。通过向第一个孔中加入 5 毫升冷冻保护剂溶液和向接下来的四个孔中加入 5 毫升 Leibovitz 15 培养基并降低冷冻保护剂浓度,开始制备六孔板。按照安全规则从液氮中取出含有卵巢皮质
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Summary
在这里,我们描述了一个协议,概述了如何从冷冻解冻的皮质组织中分离人类卵巢卵泡以进行基因表达分析。
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