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04:17 min
May 10th, 2024
DOI :
10.3791/64833-v
Chapters
0:00
Introduction
0:40
Combining Recombinase Polymerase Amplification (RPA) and CRISPR/Cas12a Systems for the Detection of DNA Viruses
1:20
CRISPR/Cas12a Detection With/Without Smartphone Microscopy (SPM)
2:23
Data Augmentation and Transfer Learning
2:58
Results: AI-Detection of DNA Virus Using RPA-CRISPR/Cas12a-SPM
3:26
Conclusion
Transcript
我们的方案引入了一种快速且高度灵敏的青蛙病毒 3 检测系统。值得注意的是,这种方法能够对DNA病毒进行即时检测,在预防大流行性疾病的发生方面发挥着至关重要的作用。该技术的主要优势在于它将RPA与CRISPR / Cas12a系统集成,并辅以基于智能手机的便携式显微镜和AI分类。
这种集成显著提高了检测效率和准确性,同时减少了人为因素造成的错误。首先,在反应缓冲液中加入四种关键的RPA酶。然后将预先设计的引物添加到混合物中。
彻底涡旋混合物。向每个 RPA 反应中加入
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Summary
我们提出了一种方案,该方案将重组酶聚合酶扩增与CRISPR / Cas12a系统相结合,用于DNA病毒的痕量检测,并构建了具有人工智能辅助分类的便携式智能手机显微镜,用于即时DNA病毒检测。
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