Transplantation of NIT-1 Cells into Non-Obese Diabetic (NOD)- Severe Combined Immunodeficient (scid) Mice
3:15
Isolation and Purification of Autoreactive Splenocytes
5:33
Intravenous Injection of Diabetic Splenocytes via the Lateral Tail Vein
7:17
In Vivo Bioluminescent Imaging of NIT-1 Grafts
8:28
Results: Monitoring Graft Survival in NOD-scid Mice by Bioluminescent Imaging
9:37
Conclusion
Transcript
This protocol can help to understand the mechanisms of protection against autoimmune attacks and identify therapeutic targets for beta cell replacement in type one diabetes. The main advantage of this technique is the relatively low cost, the simp
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This protocol describes a straightforward and minimally invasive method for transplanting and imaging NIT-1 cells in non-obese diabetic (NOD)-severe combined immunodeficient mice challenged with splenocytes purified from spontaneously diabetic NOD mice.