Production of Naive Bovine Monocyte-Derived Dendritic Cells (MoDCs)
6:01
Generation of Antigen-Pulsed MoDCs
7:20
MoDC Lymphocyte Co-Culture
8:20
Flow Cytometric Analysis
9:47
Results: In Vitro Generation of Cattle MoDCs for the Evaluation of Candidate Vaccine Antigens
12:10
Conclusion
Transcript
This protocol represents a functional in vitro assay using bovine monocyte-derived dendritic cells to measure vaccine immunogenicity prior to in vivo studies. Therefore, filling an existing gap in livestock vaccinology. It is the generation and ap
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The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle.