Fluorescence-Activated Nucleus Sorting을 사용한 지방 조직을 사용한 지방 세포 특이적 ATAC-Seq

0:51

Nucleus Isolation

2:15

Nucleus Sorting and Tn5 Tagmentation

4:08

DNA Purification and PCR Amplification

5:32

Real-Time Quantitative PCR (qPCR) Test, Additional PCR Amplification, and Second DNA Purification

6:14

DNA Fragment Size Selection

8:01

Results: Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing

10:19

Conclusion